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Snap-Omp25

Plasmid

#69599

Purpose

Snap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondria

Depositor

David Sabatini

Article

Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

Insert

Snap-Omp25

Expression

Mammalian

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-HA-hYTHDC1

Plasmid

#85167

Purpose

Expression of FLAG-HA-tagged human YTHDC1

Depositor

Samie Jaffrey

Article

Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

Insert

YTHDC1 (YTHDC1 Human)

Tags

Flag and Ha

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pET263-pUC57 24xMS2V7

Plasmid

#140705

Purpose

MS2V7 cloning

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V7

Use

Cloning vector

Mutation

24 MS2 loops V7, high-affinity C-variant, inter-s…

Promoter

None

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pPlatTET-gRNA2

Plasmid

#82559

Purpose

All in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system.

Depositor

Izuho Hatada

Article

Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

Insert

dCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CD

Expression

Mammalian

Available Since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-parkin WT

Plasmid

#45875

Depositor

Edward Fon

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin (PRKN Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE-FGFR3

Plasmid

#116742

Purpose

Lentiviral expression of FGFR3

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FGFR3 (FGFR3 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCas9-CR4

Plasmid

#62655

Purpose

Cas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetR

Depositor

Kristala Prather

Article

Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

Insert

Cas9 nuclease

Expression

Bacterial

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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EP300

Plasmid

#39018

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

EP300 (EP300 Human)

Tags

His Tev

Expression

Bacterial

Mutation

codon-optimized

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
PKA-KTR-mStrawberry reporter

Plasmid

#158688

Purpose

This plasmid is a PKA pathway reporter. It has CMV promoter driving the expression of PKA-KTR fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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Cerulean-2xFv-hRIPK3

Plasmid

#106079

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

Cerulean-2xFv-hRIPK3 (RIPK3 Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only