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pAAV.Syn.NES-jRGECO1b.WPRE.SV40

Plasmid

#100857

Purpose

AAV expression of jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRGECO1b

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-hPOMC1-26-sbGLuc-P2A-dTomato

Plasmid

#176704

Purpose

neural activity dependent release of sbGLuc from presynaptic vesicles

Depositor

Ute Hochgeschwender

Article

Prakash et al Commun Biol. 2022 Jan 11;5(1):33. doi: 1

Inserts

Gaussia luciferase, slow burn
dTomato

Use

AAV

Tags

P2A and hPOMC1-26

Promoter

hSyn

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CK(0.4)-CRY2PHR-P2A-CIBN-mCherry-VAMP2

Plasmid

#178576

Purpose

AAV vector for CK(0.4)::Opto-vTrap

Depositor

Won Do Heo

Article

Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

Insert

CRY2PHR-P2A-CIBN-mCherry-VAMP2

Use

AAV

Promoter

CamKIIa

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pXD054 KIKO CD19 CAR AAV vector

Plasmid

#192157

Purpose

CD19 CAR AAV vector

Depositor

Sidi Chen

Article

Dai et al Nat Methods. 2019 Mar;16(3):247-254. doi

Insert

anti-CD19 CAR (CD19 Human)

Use

AAV

Mutation

NA

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-DIO-Rpl22l1-3Flag-2A-eGFP-WPRE

Plasmid

#214265

Purpose

Expression of a Flag-tagged Rpl22I1 protein in cells expressing Cre recombinase allowing for the harvesting of actively transcribed mRNAs in that cell type.

Depositor

D. James Surmeier

Insert

Flag-tagged Rpl22I1 protein expressing Cre Recombinase (Rpl22 Mouse)

Use

AAV

Tags

EGFP

Expression

Mammalian

Mutation

N/A

Promoter

Synapsin

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-SomArchon

Plasmid

#126943

Purpose

Cre-dependent expression of SomArchon under CAG promoter

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.Flex.GCaMP6s.WPRE.SV40

Plasmid

#100845

Purpose

AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6s

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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RSV-Flag-Brd2

Plasmid

#86614

Purpose

Flag tagged mouse Brd2 expression construct for cell transfection

Depositor

Mario García-Domínguez

Article

Garcia-Gutierrez et al J Cell Sci. 2012 Aug 1;125(Pt 15):3671-8

Insert

Brd2 (Brd2 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

RSV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pECFP_C1_NSD2 FL

Plasmid

#221382

Purpose

Expresses NSD2 in mammalian cells

Depositor

Albert Jeltsch

Article

Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

Insert

Histone-lysine N-methyltransferase NSD2 (NSD2 Human)

Expression

Mammalian

Mutation

L237R mutation in ECFP (Please see depositor comm…

Promoter

CMV

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-STChRger2-TS-EYFP

Plasmid

#129395

Purpose

Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

soma targeted ChRger2

Use

AAV

Tags

Kv2.1-TS-EYFP

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_eCBmut

Plasmid

#164605

Purpose

Express the endocannabinoid non-responsive sensor GRAB_eCBmut in neurons

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid non-responsive sensor GRAB_eCBmut

Use

AAV

Mutation

S383T, F177A

Promoter

hSyn

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_r5-HTmut

Plasmid

#208720

Purpose

Expresses the red 5-HT sensor GRAB_r5-HTmut in neurons

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Red fluorescent 5-HT sensor GRAB_r5-HTmut

Use

AAV

Promoter

hSyn

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-SynI-CreOn-FlpOff-TeNT-P2A-EGFP

Plasmid

#176282

Purpose

Viral vector for co-expression of TeNT and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

Depositor

Martin Riccomagno

Article

Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

TeNT-P2A-EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

human Synapsin I

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLX-Z4 (GB3375)

Plasmid

#160648

Purpose

pLX series: RK2-based T-DNA binary vector for plant transformation (alpha1)

Depositor

Juan Antonio García, Fabio Pasin

Article

Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-RdLight1

Plasmid

#125708

Purpose

Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian neurons.

Depositor

Lin Tian

Article

Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

Has Service

AAV9

Insert

RdLight1

Use

AAV

Tags

Flag tag

Promoter

hSynapsin1

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP

Plasmid

#35511

Purpose

AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123T and T159C

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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WDR59 in pRK5

Plasmid

#154214

Purpose

Untagged WDR59 cDNA expression vector for transfections

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

WDR59 (WDR59 Human)

Tags

NONE

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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AAV2-hSyn-G-Flamp1-mut

Plasmid

#188570

Purpose

control probe of G-Flamp1, insensitive to cAMP

Depositor

Jun Chu

Article

Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

Insert

G-Flamp1-mut

Use

AAV

Tags

His tag

Promoter

hSyn

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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rAAV-CAG::FLEX-rev:: hM4D-2a-GFP

Plasmid

#52536

Purpose

Co-expresses hM4D and a green fluorescent label from a Cre-dependent virus

Depositor

Scott Sternson

Article

Atasoy et al Nature. 2012 Aug 9;488(7410):172-7. doi:

Has Service

AAV1

Insert

hM4D-2a-GFP (CHRM4 Human, Synthetic)

Use

AAV and Cre/Lox

Promoter

CAG

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-synP-FLEX-splitTVA-EGFP-B19G

Plasmid

#52473

Purpose

Expresses splitTVA-EGFP-B19G

Depositor

Ian Wickersham

Article

Kohara et al Nat Neurosci. 2014 Feb;17(2):269-79. doi

Has Service

AAV1

Insert

TVA-P2A-EGFP-P2A-B19G

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

Synapsin-1

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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