We narrowed to 23,831 results for: NTS;

1. pFAB1935

   Plasmid

   #47860

   Purpose

   BIOFAB reporter plasmid for measuring his_T_min_linker_crp_T_min termination efficiency

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   his_T_min_linker_crp_T_min double terminator
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   concatenate of his_min and crp_min

   Promoter

   pLTetO15

   Available Since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAT9750-BEAR-mCherry

   Plasmid

   #162990

   Purpose

   BEAR target plasmid with split mCherry and disrupted 5' splice site

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   mCherry split with an intron between amino acids 119-120
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAT15416-BEAR-mScarlet-target-BFP

   Plasmid

   #162996

   Purpose

   plasmid expressing an sgRNA targeting the BEAR-mScarlet plasmid along with a TagBFP marker

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   sgRNA targeting the BEAR-mScarlet plasmid
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-sgRNA_Dicer_2

   Plasmid

   #68808

   Purpose

   specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of

   Depositor

   Constance Ciaudo

   Article

   Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

   Insert

   sgRNA mouse Dicer1
   

   Use

   CRISPR and Mouse Targeting

   Expression

   Bacterial and Mammalian

   Available Since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440)

   Plasmid

   #160610

   Purpose

   Transcriptional unit for the expression of the AsCas12a in plant systems driven by the CaMV35s promoter.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   P35s:AsCas12a:T35s
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAT-9222

   Plasmid

   #124222

   Purpose

   Cloning plasmid for EGxFP assay with self-targeting gRNA-s

   Depositor

   Ervin Welker

   Article

   Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

   Insert

   self-targeting gRNA between EGFP halves
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. 625_HOXD13 in pmiRGlo

   Plasmid

   #78129

   Purpose

   luciferase reporter for miRNA activity

   Depositor

   Heidi Schwarzenbach

   Article

   Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

   Insert

   HOXD13 (HOXD13 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   3'UTR containing miRNA binding sites

   Promoter

   PGK

   Available Since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMOD_C2519

   Plasmid

   #91088

   Purpose

   Module C, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
   

   Use

   CRISPR

   Promoter

   OsU3

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. KHBD00638

   Plasmid

   #39705

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG3090 (Sox14 Fly)
   

   Use

   Gateway donr vector

   Promoter

   none

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

10. KHBD00118

    Plasmid

    #34272

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG7345 (Sox21a Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

11. mKO-Fibrillarin-7

    Plasmid

    #57842

    Purpose

    Localization: Nuclear - Nucleolus, Excitation: 548, Emission: 561

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Fibrillarin (FBL Human)
    

    Tags

    mKO

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCTCON2-BXG-2TAG

    Plasmid

    #158138

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the first serine residue…

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. KHBD00732

    Plasmid

    #39749

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG3848 (trr Fly)
    

    Use

    Gateway donr vector

    Promoter

    none

    Available Since

    Aug. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

14. CoV2-N-P13L

    Plasmid

    #177946

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P13L). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P13L) (N )
    

    Expression

    Mammalian

    Mutation

    P13L

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CoV2-N-P80R

    Plasmid

    #177947

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P80R) (N )
    

    Expression

    Mammalian

    Mutation

    P80R

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. CoV2-N-S235F

    Plasmid

    #177956

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S235F). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (S235F) (N )
    

    Expression

    Mammalian

    Mutation

    S235F

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. DCT.VV.Orf76

    Plasmid

    #12764

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf76
    

    Expression

    Mammalian

    Available Since

    Dec. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

18. DCT.VV.Orf98

    Plasmid

    #12786

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf98
    

    Expression

    Mammalian

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344)

    Plasmid

    #160635

    Purpose

    TU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminator

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

    Insert

    AcrIIA4
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO-sh-Myocardin

    Plasmid

    #100769

    Purpose

    Lentiviral expression of shRNA targeting MYOCD

    Depositor

    Joseph Gordon

    Article

    Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

    Insert

    Lenti-sh-Myocardin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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