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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,025 results for: 158

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Showing: 8481 - 8500 of 11025 results

  5. nLuc_AP10_PPVs_P9mS

    Plasmid
    #119302
    Purpose
    Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP10 connected with autoinhibitory coil P9mS and PPVs cleavage site in antiparallel harpin linker/for SPOC logic
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split nLuc fused to antiparallel Coiled-coil AP10 connected with P9mS via PPV cleavable linker
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119302-plasmid-map-sequence-id-232037

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  6. TFORF1471

    Plasmid
    #143814
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TOX2 (TOX2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143814-plasmid-map-sequence-id-396279

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  13. VRQR-HF1 variant

    Plasmid
    #138563
    Purpose
    Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VRQR-HF1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138563-plasmid-map-sequence-id-274789

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  14. pSL0747 (pDonor_L-MmeI)

    Plasmid
    #130647
    Purpose
    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchCAST donor DNA (L*)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Mutation
    TGTTGATG --> TGTTGGAG
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130647-plasmid-map-sequence-id-255647

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  16. pSL0746 (pDonor_R-MmeI)

    Plasmid
    #130646
    Purpose
    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated right end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchCAST donor DNA (R*)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Mutation
    TGTTGATA --> TGTTGGAA
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130646-plasmid-map-sequence-id-255642

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Showing: 8481 - 8500 of 11025 results