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5'-3xFLAG Kansl3+exon minimal CMV pCDNA5

Plasmid

#212053

Purpose

LR vector for integration of Kansl3+exon(78nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Kansl3+exon(78nt) (Kansl3 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Gse1+exon minimal CMV pCDNA5

Plasmid

#212057

Purpose

LR vector for integration of Gse1+ex(108nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Gse1+ex(108nt) (Gse1 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Kdm1a+exon minimal CMV pCDNA5

Plasmid

#212055

Purpose

LR vector for integration of Kdm1a+exon(12nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Kdm1a+exon(12nt) (Kdm1a Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Med24+exon minimal CMV pCDNA5

Plasmid

#212061

Purpose

LR vector for integration of Med24+exon(57nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Med24+exon(57nt) (Med24 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-BirA-FLAG Kdm1a-exon minimal CMV pCDNA5

Plasmid

#212088

Purpose

LR vector for integration of Kdm1a-exon into N2a FRT rtTA3 expression cells (BioID vector)

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Kdm1a-exon (Kdm1a Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-BirA-FLAG Kdm1a+exon minimal CMV pCDNA5

Plasmid

#212089

Purpose

LR vector for integration of Kdm1a+exon(12nt) into N2a FRT rtTA3 expression cells (BioID vector)

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Kdm1a+exon(12nt) (Kdm1a Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-BirA-FLAG Chtop+exon minimal CMV pCDNA5

Plasmid

#212091

Purpose

LR vector for integration of Chtop+exon(138nt) into N2a FRT rtTA3 expression cells (BioID vector)

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Chtop+exon(138nt) (Chtop Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgFAAH

Plasmid

#209197

Purpose

Mutagenesis of Faah

Depositor

Larry Zweifel

Article

Balsevich et al Elife. 2023 Apr 11;12:e81919. doi: 10.75

Insert

Faah (Faah Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Promoter

CMV

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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Bn R52A, R54A pMT2

Plasmid

#206087

Purpose

Cav2.2 N-terminus (amino acids 1-95) with R52A and R54A mutations that prevent dominant-negative suppression of Cav2.2 currents by truncated Cav2.2

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

R52A, R54A

Promoter

Ad MLP/TPL/SV40

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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An HA CAAX pMT2

Plasmid

#206116

Purpose

expression of Cav2.1 N-terminus (amino acids 1-100) with a C-terminal CAAX (last 10aa of H.Ras) motif and a HA tag

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Promoter

Ad MLP/TPL/SV40

Available Since

Nov. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Bn CAAX pcDNA3

Plasmid

#206088

Purpose

Cav2.2 N-terminus (amino acids 1-95) with a C-terminal tag of the final 10 aa of H.Ras containing CAAX motif

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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An CAAX pMT2

Plasmid

#206118

Purpose

expression of Cav2.1 N-terminus (amino acids 1-100) with C-terminal CAAX (last 10 aa of H.Ras) motif

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Promoter

Ad MLP/TPL/SV40

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 W391A pcDNA3

Plasmid

#206085

Purpose

expression of rabbit Cav2.2 calcium channel with a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

Depositor

Annette Dolphin

Article

Leroy et al J Neurosci. 2005 Jul 27;25(30):6984-96.

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pCas_4(I-U)/1_2_VB

Plasmid

#186445

Purpose

Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B). Cas4 domain swapped with type U-I Cas4 domain from G. sulfurreducens

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1 and Cas2

Use

CRISPR

Mutation

Cas4 domain swapped with Cas4 domain from G. sulf…

Promoter

T7

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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ApoE3_D125I_47-300_mCh-SspB (pBS1071)

Plasmid

#185294

Purpose

For the mammalian expression of the human protein ApoE3_D125I_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3_D125I_47-300

Expression

Mammalian

Mutation

D125I

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4 TAPBPR-TM-TN6

Plasmid

#178650

Purpose

Mammalian expression of FLAG-tagged TAPBPR with MHC TM (E205K, R207E, Q209S, Q272S)

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

TAPBPR (TAPBPL Human)

Tags

FLAG and Signal/leader sequence from HLA class I …

Expression

Mammalian

Mutation

E205K, R207E, Q209S, Q272S, switched transmembran…

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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CAHS2_PARRC_98-130_mCh-SspB (pBS1077)

Plasmid

#185300

Purpose

For the mammalian expression of the tardigrade protein CAHS2_PARRC_98-130 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS2_PARRC_98-130

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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DSUP_RAMVA_1-208_mCh-SspB (pBS1073)

Plasmid

#185296

Purpose

For the mammalian expression of the tardigrade protein DSUP_RAMVA_1-208 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DSUP_RAMVA_1-208

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE3_47-300_mCh-SspB (pBS1070)

Plasmid

#185293

Purpose

For the mammalian expression of the human protein ApoE3_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3_47-300

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE4_47-300_mCh-SspB (pBS1069)

Plasmid

#185292

Purpose

For the mammalian expression of the human protein ApoE4_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE4_47-300

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge