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pCA15-Ubc-NLS- scFV-tdTomato

Plasmid

#199446

Purpose

Expression of scFV-tdTomato that binds to the GCN4 peptide from the SunTag System in mammalian cells

Depositor

Baohui Chen

Article

Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

Insert

scFV-tdTomato

Use

Lentiviral

Expression

Mammalian

Promoter

human ubiquitin C (UBC)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pJM195A

Plasmid

#218125

Purpose

Hybrid circuit displaying hsCRBN-CTD bait and driving expression of gIII

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

CRBN_CTD
M13 pIII

Tags

434cI_RR69

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJM194A

Plasmid

#218124

Purpose

Hybrid circuit displaying hsCRBN-CTD bait and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

CRBN_CTD
luxAB

Tags

434cI_RR69

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(TIGIT)

Plasmid

#177902

Purpose

Bacterial expression of his10-tagged human TIGIT Intracellular Domain

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

TIGIT (aa 163-244 only) (TIGIT Human)

Tags

10xHis-Thrombin cut site-T7 tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pLHCX-Flag-mPKM2(K433E)

Plasmid

#42514

Depositor

Dimitrios Anastasiou

Article

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

Pyruvate Kinase M2 Lys433->Glu

Use

Retroviral

Tags

Flag

Mutation

Changed lysine 433 to glutamate

Available Since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
pLV-RhoB Sensor-IRES-Blast

Plasmid

#222633

Purpose

Rho reporter

Depositor

Arnold Hayer

Article

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

RhoB Sensor

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-SuperNovaGreen-T2A-dTomato

Plasmid

#193798

Purpose

Expresses SuperNova Green in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

SuperNova Green

Use

AAV

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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ZJOM100

Plasmid

#133662

Purpose

Integration of MNN9-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-MNN9-mTagBFP2 (MNN9 Budding Yeast)

Tags

mTagBFP2

Expression

Yeast

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMIG-Flag-RIPK1-wt

Plasmid

#102677

Purpose

retroviral expression plasmid for RIPK1-wt

Depositor

Hasam Habelhah

Article

Zhang et al Mol Cell Biol. 2015 Oct;35(19):3324-38.

Insert

RIPK1 (RIPK1 Human)

Use

Retroviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Promoter

unknown

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pACYC-Cas12g1-noncoding

Plasmid

#120880

Purpose

Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12g1 system in the native genomic loci; expresses the tracrRNA required for activity.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Noncoding sequences for CRISPR-Cas12g1

Use

CRISPR

Expression

Bacterial

Promoter

lac

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-SFB-RNF169

Plasmid

#99392

Purpose

Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged RNF169

Depositor

Michael Huen

Article

Chen et al J Biol Chem. 2012 Aug 10;287(33):27715-2

Insert

RNF169 (RNF169 Human)

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe-Sik-WT

Plasmid

#210722

Purpose

Construct for stable expression of mouse wild type Ptk6 (Sik) in mammalian cells.

Depositor

Angela Tyner

Article

Derry et al Mol Cell Biol. 2000 Aug;20(16):6114-26.

Insert

Protein tyrosine kinase 6 (Ptk6 Mouse)

Expression

Mammalian

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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ALIX(P801G)-Strep

Plasmid

#180024

Purpose

Codon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

ALIX(1-868, P801G)-Strep (PDCD6IP Human)

Tags

Strep tag (WSHPQFEK)

Expression

Bacterial

Mutation

P801G

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-BaLgp160-754*

Plasmid

#123212

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate; C-terminal truncation

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Premature stop co…

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-TTF-1-HDD

Plasmid

#52063

Purpose

It encodes a mutant of TTF-1 in which the homeodomain is deleted

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

Thyroid Transcription Factor 1 (NKX2-1 Human)

Expression

Mammalian

Mutation

Homeodomain deleted

Available Since

April 11, 2014

Availability

Academic Institutions and Nonprofits only
pSLIK_hygro_3xflag_Luciferase

Plasmid

#136528

Purpose

Lentiviral expression vector for an inducible 3xflag-tagged Luciferase

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

Luciferase

Use

Lentiviral; Destinatioin vector for gateway cloni…

Tags

3x FLAG

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pgRNA_hMECP2_promoter_No3

Plasmid

#194898

Purpose

Lentiviral construct with mCherry and Puro cassettes to express sgRNA-3 targeting the promoter of human MECP2

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Insert

sgRNA targeting human MECP2 promoter No.3

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pgRNA_hMECP2_promoter_No9

Plasmid

#194903

Purpose

Lentiviral construct with mCherry and Puro cassettes to express sgRNA-9 targeting the promoter of human MECP2.

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Insert

sgRNA targeting human MECP2 promoter No.9

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pQTEV-GYG

Plasmid

#31324

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

glycogenin (GYG1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNA500

Plasmid

#149583

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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