We narrowed to 17,096 results for: Por

1. pENTR-TMEM144

   Plasmid

   #221426

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   TMEM144 (TMEM144 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-Sensor_P2A_SLC2A4

   Plasmid

   #221429

   Purpose

   Gateway entry clone encoding a polyprotein that is cleaved to FRET glucose sensor and SLC2A4 proteins

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   Sensor_P2A_SLC2A4 (SLC2A4 Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-3xFlag-SPNS1

   Plasmid

   #198568

   Purpose

   SPNS1 codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1 with n-terminal 3xFlag (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. SSH-pSUMO-β′-COPI-WD40-Tyr33Ala

   Plasmid

   #208170

   Purpose

   Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Tyr33Ala mutant (residues 1-301)

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

   Insert

   N-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Tyr33Ala substitution
   

   Use

   Tags

   His-tag, SUMO-tag, and Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Glu

   Plasmid

   #218461

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Glu substitution)

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

   Insert

   SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Glu substitution (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Thr1273AGlu substitution

   Promoter

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Asp

   Plasmid

   #218462

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Asp substitution)

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

   Insert

   SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Asp substitution (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Thr1273Asp substitution

   Promoter

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-RepoMan-RATA

   Plasmid

   #184270

   Purpose

   Expresses shRNA resistant GFP-RepoMan RATA Mutant

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Insert

   cell division cycle associated 2 (CDCA2 Human)
   

   Use

   Tags

   3xHA and GFP

   Expression

   Mammalian

   Mutation

   V383A, F395A

   Promoter

   CMV

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-RepoMan-T394A

   Plasmid

   #204994

   Purpose

   Expresses shRNA resistant GFP-RepoMan T394A Mutant

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Insert

   cell devision cycle associated 2 (CDCA2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed Threonine 394 to Alanin

   Promoter

   CMV

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. ENTR_BN001T-N_START

   Plasmid

   #218058

   Purpose

   RESOLUTE SLC binder cloning plasmid

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

   Insert

   BN001T-N_START
   

   Use

   Cloning plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. ENTR_BN006A-P_START

    Plasmid

    #218059

    Purpose

    RESOLUTE SLC binder cloning plasmid

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

    Insert

    BN006A-P_START
    

    Use

    Cloning plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. ENTR_BN001M-T_START

    Plasmid

    #218060

    Purpose

    RESOLUTE SLC binder cloning plasmid

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

    Insert

    BN001M-T_START
    

    Use

    Cloning plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. plentiCRISPR-v2 Puro FLVCR1_sg5

    Plasmid

    #218522

    Purpose

    sgRNA targeting human FLVCR1

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    FLVCR1 (FLVCR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. plentiCRISPR-v2 Puro CHKA_sg2

    Plasmid

    #218523

    Purpose

    sgRNA targeting human CHKA

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    CHKA (CHKA Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. plentiCRISPR-v1 GFP FLVCR1_sg5

    Plasmid

    #218521

    Purpose

    sgRNA targeting human FLVCR1

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    FLVCR1 (FLVCR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. B2M-SDMutation-gRNA

    Plasmid

    #215547

    Purpose

    gRNA targeting B2M to introduce splice donor mutation on the first intron of the locus

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    B2M (B2M Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a-SRRD-V5-APEX2 (in pLEX_307)

    Plasmid

    #214921

    Purpose

    constitutive expression of SRRD fused to V5 and APEX2 - used for APEX2 proximity biotinylation

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    SRRD (SRRD Human)
    

    Use

    Lentiviral

    Tags

    V5-APEX2

    Expression

    Mutation

    Promoter

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET SUMO-Doc2β C2AB (G361C)

    Plasmid

    #213631

    Purpose

    Bacterial expression of SUMO-tagged rat Doc2β C2AB (G361C)

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    Doc2β C2AB (G361C) (Doc2b Rat)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    G361C, all original cysteines mutated to alanines

    Promoter

    Available Since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. INS-3'gRNA

    Plasmid

    #210468

    Purpose

    gRNA targeting INS 3' terminal for CRISPR-Cas9-mediated knock-in

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    INS (INS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.3R/MPL-shRNA4

    Plasmid

    #204529

    Purpose

    Lentiviral expression of MPL-shRNA4; gene knock down of human MPL

    Depositor

    Norio Komatsu

    Article

    Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

    Insert

    MPL-shRNA4 (MPL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.3R/MPL-shRNA3

    Plasmid

    #204528

    Purpose

    Lentiviral expression of MPL-shRNA3; gene knock down of human MPL

    Depositor

    Norio Komatsu

    Article

    Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

    Insert

    MPL-shRNA3 (MPL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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