169,450 results
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Plasmid#190664PurposeExpresses U6-driven N7 sgRNA2 (poly-T mutated out), BsmbI dropout cassette for cloningDepositorInsertN7 sgRNA2
ExpressionMammalianPromoterU6Available SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-PGK-puromycin
Plasmid#51133Purposeexpresses sgRNA from the U6 promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6Available SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG-SsAPOBEC3B
Plasmid#198536PurposeExpresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-SsAPOBEC3B
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGolden-AAV
Plasmid#51424PurposeContain AAV ITRs, lacZ expressing for blue-white selection for golden-gate reactionDepositorInsertlacZ
UseAAVPromoterlacZAvailable SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTP396
Plasmid#149336PurposeBacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobodyDepositorInsertanti-GFP nanobody
TagsBiotin acceptor peptide (Avi-tag), His14, and SUM…ExpressionBacterialAvailable SinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-tdTomato (AAV2)
Viral Prep#28306-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomato (Cre-dependent)Available SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-EGFP-GRAM-W
Plasmid#211704PurposeLentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (pLJM1-EGFP-GRAM1b G187W)DepositorInsertEGFP-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
UseLentiviralTagsEGFPExpressionMammalianMutationChanged Glycine 187 to TryptophanPromoterCMVAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)
Plasmid#199605PurposeExpression of cell permeable opAsCas12a in E. Coli.DepositorInsertopCas12a-T8N
UseCRISPRTags6xHis, Twin-strep, SUMOExpressionBacterialAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-LacI-mHP1a
Plasmid#232637Purposelac repressor (LacI) which binds lacO sites; labeled with HaloTag and coupled with effector protein mouse HP1alphaDepositorAvailable SinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMMK805
Plasmid#207139PurposeAssembled Tn10 with ptac promoter driving gfpDepositorInsertTn10-Ptac-GFP
UseSynthetic BiologyExpressionBacterialPromoterptacAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only