We narrowed to 19,051 results for: Tec

1. pNB_EA_R3tdTomatoi2

   Plasmid

   #185969

   Purpose

   Expresses tdTomato from PR promoter with optimized RBS (i2) in E.coli Dh5alpha cell. The construct is in reverse direction network brick.

   Depositor

   Sangram Bagh

   Article

   Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

   Insert

   Pr-i2rbs-tdTomato cassette in reverse direction in Network Brick
   

   Use

   Synthetic Biology

   Promoter

   PR

   Available Since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pv6_CBX7_Chromo-W35A_TAF3_Phd

   Plasmid

   #179401

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo(W35A) - TAF3_Phd

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX7_Chromo(W35A) - TAF3_Phd
   

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   Chromodomain W35A

   Promoter

   CAGGS

   Available Since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDARMO_CMVT_3xFLAG-HUWE1_delta169-189

   Plasmid

   #187135

   Purpose

   mammalian cell expression of 3xFLAG-HUWE1 delta169-189

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   delta169-189

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDARMO_CMVT_3xFLAG-HUWE1_delta3896-3951

   Plasmid

   #187133

   Purpose

   mammalian cell expression of 3xFLAG-HUWE1 delta3896-3951

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   delta3896-3951

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDARMO_CMVT_3xFLAG-HUWE1_I3969A/F3982A

   Plasmid

   #187129

   Purpose

   mammalian cell expression of 3xFLAG-HUWE1 I3969A/F3982A

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   I3969A/F3982A

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221-HUWE1_UBA/UIM/UBM-mut

   Plasmid

   #187153

   Purpose

   Entry clone

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Entry clone

   Mutation

   L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIG-742pUC19-tNGFR-P2A-Caspase8

   Plasmid

   #186099

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   CASPASE8
   

   Use

   CRISPR

   Mutation

   WT with tNGFR sequence

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTS1043

   Plasmid

   #169621

   Purpose

   Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA) .

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
   

   Expression

   Mammalian

   Promoter

   PhCMV / PPGK

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. BMP10 N67Q N131Q D4G (S2-2)

   Plasmid

   #184622

   Purpose

   for transfection into S2 cells and expression of BMP10 protein

   Depositor

   Timothy Springer

   Article

   Le et al J Mol Biol. 2022 Mar 15;434(5):167439. d

   Insert

   BMP10 (BMP10 Human)
   

   Tags

   8x His - streptavidin binding peptide

   Expression

   Insect

   Mutation

   N67Q, N131Q, ARIRR(312-316) replaced with G

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTS2361_Tier3(SB)-rtTA-YPet_PuroR

    Plasmid

    #169657

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    

    Expression

    Mammalian

    Promoter

    PRPBSA

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTS1042

    Plasmid

    #169620

    Purpose

    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Expression

    Mammalian

    Promoter

    PhCMV / PPGK

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTS2308-Tier1-pGL3-PPGK-NLuc_TB

    Plasmid

    #169551

    Purpose

    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    pGL3-insulator-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    

    Expression

    Mammalian

    Promoter

    PPGK

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTS2363_Tier3(PB)-rtTA-YPet_PuroR

    Plasmid

    #169659

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    

    Expression

    Mammalian

    Promoter

    PRPBSA

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pnEA-NpM-DmThor_50-83-R56ER63E-GB1_AA

    Plasmid

    #148272

    Purpose

    Bacterial Expression of DmThor_50-83-R56ER63E

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    DmThor_50-83-R56ER63E (Thor Fly)
    

    Expression

    Bacterial

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-U6-UL29-U3-UL8

    Plasmid

    #166685

    Purpose

    To express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (non-EGFP version).

    Depositor

    Yujia Cai

    Article

    Yin et al Nat Biotechnol. 2021 Jan 11. pii: 10.103

    Insert

    UL8 and UL29 gRNA (UL29, UL8 HSV-1)
    

    Use

    Lentiviral

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEZT-GST-SLFN2-T1

    Plasmid

    #174320

    Purpose

    Expression of recombinant GST-SLFN2-R67A, K68A, N71A

    Depositor

    Bruce Beutler

    Article

    Yue et al Science. 2021 May 14;372(6543). pii: 372

    Insert

    GST-SLFN2-R67A, K68A, N71A (Slfn2 Mouse)
    

    Use

    Bacmam, baculovirus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEZT-GST-SLFN2-T2

    Plasmid

    #174321

    Purpose

    Expression of recombinant GST-SLFN2-K242A, K249A, Q301A

    Depositor

    Bruce Beutler

    Article

    Yue et al Science. 2021 May 14;372(6543). pii: 372

    Insert

    GST-SLFN2-K242A, K249A, Q301A (Slfn2 Mouse)
    

    Use

    Bacmam, baculovirus

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV_eA3A T31A-nCas9

    Plasmid

    #163538

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A T31A-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)eA3A T31A

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV_UdgX-EE-UdgX-nCas9

    Plasmid

    #163564

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-BRCA1-RNF169UBD

    Plasmid

    #119009

    Purpose

    Mammalian expression of a fusion protein of human BRCA1 with the human RNF169 Ubiquitin binding domain and BFP expression

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    BRCA1 (BRCA1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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