We narrowed to 8,384 results for: Dos
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Plasmid#69622PurposeThe heterotrimeric G-protein subunit, Gαi isoform 3, tagged with mTurquoise2DepositorInsertGalphai3-mTurquoise2
TagsmTurquoise2ExpressionMammalianPromoterCMVAvailable SinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-Mac 3.0-EYFP
Plasmid#35515PurposeLentiviral expression of CaMKIIa-driven Mac 3.0 for optical inhibition.DepositorInserteMac 3.0-EYFP
UseLentiviralTagsEYFPExpressionMammalianMutationTrafficking signal, ER exportPromoterCaMKIIaAvailable SinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-SARS-CoV-2-3CL
Plasmid#160278PurposeAllows for constituitive mammalian expression of the SARS-CoV-2 3CL protease via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pF CAG luc puro
Plasmid#67501PurposeConstitutive expression of firefly luciferase in mammalian cellsDepositorInsertFirefly luciferase
UseLentiviral and LuciferaseExpressionMammalianPromoterCAGAvailable SinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-SpyCatcher-ELP-GFP
Plasmid#69835PurposeBacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.DepositorInsertSpyCatcher-ELP-GFP
Tags6x His Tag, GFP, and TEV TagExpressionBacterialPromoterT5 promoter/lac operator elementAvailable SinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
Plasmid#62373PurposeExpression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cellsDepositorInsert(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
Tags(-30)dGFP, 6xHis tag, Cathepsin cleavage site, an…ExpressionBacterialMutationY67S amino acid substitution in (-30)GFP renders …PromoterT7Available SinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX311-Cas9
Plasmid#118018PurposeConstitutive expression of Cas9DepositorInsertCas9
UseLentiviralExpressionMammalianPromoterEF1alphaAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-5'-ENGRAM
Plasmid#179157PurposepiggyBac plasmid for 5' ENGRAM recorderDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionMammalianPromoterminPAvailable SinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Galphai2-mTurquoise2
Plasmid#69621PurposeThe heterotrimeric G-protein subunit, Gαi isoform 2, tagged with mTurquoise2DepositorInsertGalphai2-mTurquoise2
TagsmTurquoise2ExpressionMammalianPromoterCMVAvailable SinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
TGFbetaR1 (T204D)-pcw107-V5
Plasmid#64629Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertTGFBR1 (transcript variant 1) (TGFBR1 Human)
UseLentiviralTagsV5MutationT204DPromoterPGKAvailable SinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroR-T2A-BFP
Plasmid#155307PurposeRfx Cas13d guide cloning lentiviral vector with constitutively expressed puromycin resistance 2A-tagged with tagBFPDepositorInsertRfx Cas13d CRISPR RNA puromycin resistance 2A-tagged BFP
UseLentiviralExpressionMammalianPromoterhU6, EF1aAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDest-Sumo
Plasmid#106980PurposeLR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tagDepositorTypeEmpty backboneTagsHis8-SumoExpressionBacterialPromoterT7Available SinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-Dnmt3a3L-sc27
Plasmid#71827PurposeFor bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.DepositorInsertDnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
Tags6xHis tagExpressionBacterialAvailable SinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS306-URA3-PHIS3-PfV-GS-Sapphire
Plasmid#116932PurposeYeast integrative vector for the exression of the 40nm GEM under the HIS3 promoterDepositorInsertPfv
TagsSapphireExpressionYeastPromoterHIS3Available SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY027
Plasmid#84742PurposeExpresses huLbCpf1 and crRNA guideDepositorInserthuLbCpf1
UseCRISPRTags3xHA and NLSExpressionMammalianPromoterCMVAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
Kras (G12V)-pcw107
Plasmid#64602Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.SF-iGluSnFR.A184S
Plasmid#106198PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184S
UseAAVMutationGltI: A184SPromoterCAGAvailable SinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 puro
Plasmid#104994PurposeThis 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralExpressionMammalianAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
p_m16S
Plasmid#160549PurposeUsed for mycoplasma detection by qPCR. 16S insert truncated internally from the strain Mycoplasma capricolum subsp. capricolum strain California Kid. ATB: CarbenicillineDepositorInsert16S rDNA fragment from M. capricolum subsp. capricolum strain California Kid (gi_83283139)
UseUnspecifiedAvailable SinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
myr-FLAG-AKT1-pcw107
Plasmid#64606Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only