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Plasmid#189742PurposeThe construct was used to express GFP tagged human ALDH1A1 in mammalian cells for the analysis of the subcellular location of this protein.DepositorInsertALDH1A1 (ALDH1A1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterPGKAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-PYL1-sfGFP-Coilin
Plasmid#122177Purposeexpression of PYL1-SfGFP-Coilin, lentivirus vectorDepositorInsertCoilin (COIL Human)
UseLentiviralTagsPYL1-SFGFPExpressionMammalianMutationPromoterPGKAvailable sinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-EGFP-ALDH1A2-Neo
Plasmid#189743PurposeThe construct was used to express GFP tagged human ALDH1A2 in mammalian cells for the analysis of the subcellular location of this protein.DepositorInsertALDH1A2 (ALDH1A2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterPGKAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Venus-Fluc (puro)
Plasmid#140328PurposeLentivirus for expression of Venus-Fluc (firefly luciferase) fusion proteinDepositorInsertVenus-Firefly Luciferase (luc2)
UseLentiviralTagsExpressionMutationPromoterhuman PGK promoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK GFP Blast (w510-5)
Plasmid#19069Purpose3rd gen lentiviral eGFP expression vector, PGK promoter, BlastDepositorInsertenhanced green fluorescent protein
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Neo DEST (w531-1)
Plasmid#19067Purpose3rd gen lentiviral Gateway destination vector, expression, PGK promoter, NeoDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W
Plasmid#112927PurposeCRISPR-a reporter assay lentiviral vector (gTetO)DepositorInserthU6-gRNASAM(gTetO), TREGFP, PGKpuroGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGKCMV)-nucTomato-hygro
Plasmid#208573PurposeLV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an PGK/CMV promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmnucTomato
d2nucEGFP
HygroR
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterPGK/CMV and minPAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK V5-LUC Puro (w543-1)
Plasmid#19360Purpose3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, PuroDepositorInsertFirefly Luciferase
UseLentiviral and LuciferaseTagsV5ExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (HNF1A)
Plasmid#188384PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factorDepositorInsertPGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-dCas9-KRAB-PGK-HygR
Plasmid#83890PurposeLentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.DepositorInsertshumanized dead Cas9 KRAB
aminoglycoside phosphotransferase from E. coli
UseCRISPR and LentiviralTagsFlagExpressionMutationD10A and H840APromoterHuman Ubiquitin C Promoter and mouse phosphoglyce…Available sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTS2303-Tier1-CoTC-PPGK-NLuc_TB
Plasmid#169548PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertCoTC-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-HA-CRBN-pGK-HYG
Plasmid#107378PurposeLentiviral expression of HA-CRBNDepositorInsertcereblon (CRBN Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterUbcPAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Tfe3-pgk-hph
Plasmid#48755PurposePiggyBac backbone, CAG promoter driving murine Tfe3, Hygromycin selectionDepositorInsertTfe3 (Tcfe3 Mouse)
UsePiggybacTagsExpressionMutationPromoterCAGAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSCV(gfp)U6sgRNA5(BbsI)-PGKpuroBFP
Plasmid#102798PurposeRetrovirus for delivery of one sgRNA (self-targeting) - GFP-targeting control plasmid contains GFP instead of PuroRDepositorInsertssgRNA
EGFP
UseCRISPR and RetroviralTagsExpressionMammalianMutationH231LPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W
Plasmid#112926PurposeCRISPR-a reporter assay lentiviral vector (empty gRNA control)DepositorInserthU6-gRNASAM(empty), TREGFP, PGKpuroGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Blast DEST (w524-1)
Plasmid#19065Purpose3rd gen lentiviral Gateway destination vector, expression, PGK promoter, BlastÂDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Blast V5-LUC (w528-1)
Plasmid#19166Purpose3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, BlastDepositorInsertFirefly Luciferase
UseLentiviral and LuciferaseTagsV5ExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHR-Hsp-eGFP-PGK-mCherry
Plasmid#191862PurposeHeat inducible eGFP reporter with constitutive mCherry markerDepositorInsertseGFP
mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterHSPA7 promoter and PGK promoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2/CMV-Luc/PGK-SB100X
Plasmid#192869PurposeCarries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.DepositorInsertsluc
SB100X
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only