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pCMV-T7-hAcrVA2.1-NLS(sv40) (BPK5059)

Plasmid

#115137

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA2.1 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA2.1 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pC010 LshCas13a locus with nontargeting spacer

Plasmid

#91901

Purpose

LshCas13a locus into pACYC184 with nontargeting spacer

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

Insert

LshCas13a locus with non-targeting spacer

Expression

Bacterial

Available Since

Oct. 16, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-mGrn-myc

Plasmid

#178944

Purpose

Construct for expressing murine progranulin (Grn) in AAV

Depositor

Erik Roberson

Article

Arrant et al Brain. 2017 May 1;140(5):1447-1465. doi:

Insert

Grn (Grn Mouse)

Use

AAV

Tags

Myc

Promoter

CBA

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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TAL2272

Plasmid

#36709

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

KRAS-TALEN_Left (Kras Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2273

Plasmid

#36710

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

KRAS-TALEN_Right (Kras Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-T7-hAcrVA3.1-NLS(sv40) (RTW2624)

Plasmid

#115139

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA3.1 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA3.1 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)

Plasmid

#115140

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA3 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)

Plasmid

#115138

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA2 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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TAL2238

Plasmid

#36689

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CHD7-TALEN_Left (Chd7 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2239

Plasmid

#36690

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CHD7-TALEN_Right (Chd7 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
Zebrafish-gRNA-0001

Plasmid

#42241

Purpose

gRNA targeted to zebrafish gene apoea

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

gRNA-apoea (apoea Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

T7

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only
AtS/MAR10 insulator sequence (GB3458)

Plasmid

#160638

Purpose

pEGB3a1 AtS/MAR10

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

AtS/MAR10 insulator sequence

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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TAL2317

Plasmid

#36743

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FGFR1-TALEN_Right (FGFR1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2316

Plasmid

#36742

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FGFR1-TALEN_Left (FGFR1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only
pGWKS6

Plasmid

#139413

Purpose

Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mMaroon1 marker.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

mMaroon1

Expression

Yeast

Promoter

TEF1

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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Zebrafish-gRNA-0002

Plasmid

#42242

Purpose

gRNA targeted to zebrafish gene drd3

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

gRNA-drd3 (drd3 Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

T7

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only
pYSD344

Plasmid

#253044

Purpose

Encodes 2xFLAG-tagged Aga2 with Protein A ZZ domain as a C-terminal fusion in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker

Depositor

Paul Young

Article

Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102

Insert

Aga2 Protein A

Expression

Yeast

Available Since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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pYSD728

Plasmid

#253050

Purpose

Encodes OST1 Sig Pep, LC Cutinase-WT, FLAG-tagged SED1 surface display anchor in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker

Depositor

Paul Young

Article

Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102

Insert

Leaf compost cutinase

Expression

Yeast

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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pYSD373

Plasmid

#253048

Purpose

Encodes an AGA2 Signal Peptide, Anti-mCherry_nanobody_LaM-4 and 2xFLAG-tagged-649-stalk anchor in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker

Depositor

Paul Young

Article

Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102

Insert

Anti-mCherry_LaM-4 nanobody 649-stalk anchor

Expression

Yeast

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pYSD281

Plasmid

#253022

Purpose

Encodes the Suc2 signal peptide, Invertase, C-terminal GFP11-M3 and 6xHistidine tags in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker

Depositor

Paul Young

Article

Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102

Insert

Suc2

Expression

Yeast

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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