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We narrowed to 1,268 results for: grna cloning vector

Showing: 841 - 860 of 1268 results

  1. pEJS1830 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-U6-Fah

    Plasmid
    #199263
    Purpose
    Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting the point mutation in the mouse Fah gene of a HT1 mouse model
    Depositor
    Erik Sontheimer
    Article
    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.
    Insert
    NmeABE8e
    Use
    AAV and CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    U1a
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    199263-plasmid-map-sequence-id-389813

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  2. pEJS-HZ79 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26_V2

    Plasmid
    #199261
    Purpose
    Optimized single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene. This optimized construct showed improved in vivo editing efficiency.
    Depositor
    Erik Sontheimer
    Article
    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.
    Insert
    NmeABE8e
    Use
    AAV and CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    U1a promoter
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    199261-plasmid-map-sequence-id-389819

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  5. pSECC

    Plasmid
    #60820
    Purpose
    3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre
    Depositor
    Tyler Jacks
    Article
    Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature
    Inserts
    Cas9
    Cre
    Use
    CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    BsmBI site eliminated by C->A; E308G
    Promoter
    EFS and EFS (after Cas9-2A)
    Available since
    Nov. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    60820-plasmid-map-sequence-id-371636

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  11. MCS_BsmBI

    Plasmid
    #241170
    Purpose
    Middle entry vector for mini-Golden subcloning platform; MCS; BsmBI
    Depositor
    Junsu Kang
    Insert
    MCS; BsmBI
    Use
    Mini-golden
    Tags
    Expression
    Mutation
    TCGC linker
    Promoter
    Available since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241170-plasmid-map-sequence-id-482039

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  12. memb-mNeongreen

    Plasmid
    #241175
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Tags
    Expression
    Mutation
    TCGC linker
    Promoter
    Available since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241175-plasmid-map-sequence-id-482019

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  14. pcDNA3.1-CRISPR-Puro

    Plasmid
    #208646
    Purpose
    pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Clawed frog nucleoplasmin NLS, Flag, Puromycin re…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6 in tandem
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208646-plasmid-map-sequence-id-418362

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  16. SA-memb-mNeongreen

    Plasmid
    #241207
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Tags
    Expression
    Mutation
    TCGC linker
    Promoter
    Available since
    Aug. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    241207-plasmid-map-sequence-id-482175

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  17. pMid-TCGC-mNeongreen

    Plasmid
    #241171
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Tags
    Expression
    Mutation
    TCGC linker
    Promoter
    Available since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241171-plasmid-map-sequence-id-482081

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  18. lifeact-mNeongreen-pA

    Plasmid
    #241202
    Purpose
    Middle entry vector for mini-Golden subcloning platform; lifeact-mNeongreen
    Depositor
    Junsu Kang
    Insert
    lifeact-mNeongreen
    Use
    Mini-golden
    Tags
    Expression
    Mutation
    TCGC linker
    Promoter
    Available since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241202-plasmid-map-sequence-id-482031

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  19. linker-P2A-mNeongreen-ORF1

    Plasmid
    #241176
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Tags
    Expression
    Mutation
    TCGC linker
    Promoter
    Available since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241176-plasmid-map-sequence-id-482017

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  20. linker-P2A-mNeongreen-ORF2

    Plasmid
    #241177
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Tags
    Expression
    Mutation
    TCGC linker
    Promoter
    Available since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241177-plasmid-map-sequence-id-482016

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Showing: 841 - 860 of 1268 results