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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,215 results for: spl

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Showing: 841 - 860 of 4215 results

  7. PB-CAG-dCasRx-RBM25-IRES-BleoR

    Plasmid
    #221002
    Purpose
    Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Publication
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    dCasRx-RBM25-IRES-BleoR
    Use
    CRISPR
    Tags
    NLS and NLS-HA
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    221002-plasmid-map-sequence-id-436162

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  12. pJYDNg

    Plasmid
    #162452
    Purpose
    Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin. The plasmid is carrying the eUnaG2 fluorescent protein and long 2G linker.
    Depositor
    Gideon Schreiber
    Publication
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    Ha and myc and eUnaG2 fluorescent protein
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162452-plasmid-map-sequence-id-321450

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  16. SpyTag-sfGFP

    Plasmid
    #184226
    Purpose
    Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.
    Depositor
    Mark Howarth
    Publication
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpyTag-sfGFP
    Tags
    His6, SpyTag, and TEV protease cleavage site
    Expression
    Bacterial
    Promoter
    T5
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184226-plasmid-map-sequence-id-361605

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  19. TDP-REGv2(mScarlet-FLAG)#11 in pTwist-CMV backbone

    Plasmid
    #216155
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Very bright and decent dynamic range (>10-fold), slightly leaky. It uses a single intron. [Code 'B12']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Publication
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic splice site and C-terminal FLAG tag
    Expression
    Mammalian
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216155-plasmid-map-sequence-id-437763

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Showing: 841 - 860 of 4215 results