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pCMV-eNme2-T.1-ABE8e

Plasmid

#185668

Purpose

Expresses eNme2-T.1-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-neNme2-T.2

Use

CRISPR

Expression

Mammalian

Mutation

Nme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H…

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pFB2D

Plasmid

#195286

Purpose

Bicistronic DTR-EGFP fusion and Puromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

DTR EGFP 2A Puro

Expression

Mammalian

Promoter

EFS

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-monSTIM1

Plasmid

#161795

Purpose

Mammalian expression plasmid of monSTIM1 (ultra-photosensitive optogenetically-activated STIM1 protein)

Depositor

Won Do Heo

Article

Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:

Insert

MonSTIM1 (STIM1 Human, Mustard Weed)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-E1MLS-2xMyc-Ku-IRES-Hygro

Plasmid

#234950

Purpose

Human codon optimized Ku (Mycobacterium) with N-terminal E1 MLS expressing plasmid

Depositor

Agnel Sfeir

Article

Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

human codon optimized Ku with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Promoter

EF1a

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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R619-M66-303: CMV51p> HCoV-OC43 S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166015

Purpose

mammalian expression of HCoV-OC43 soluble spike trimer protein

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

HCoV-OC43 S (spike)

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pKL038 Lenti U6 dCas12a gRNA Puro mCherry

Plasmid

#195546

Purpose

dCas12a gRNA expression backbone

Depositor

Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti U6- empty cassette_Direct repeat_puro_mcherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-EF1a-D614G-N501Y

Plasmid

#177939

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Spike protein (D614G, N501Y) (S )

Expression

Mammalian

Mutation

D614G, N501Y

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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MT-AvrXa10-FT

Plasmid

#234373

Purpose

T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-GFP-AvrXa10-GB1) with optimized activation domain (AvrXa10), targeting Arabidopsis FT locus

Depositor

Michael Smanski

Article

Zinselmeier et al Plant Biotechnol J. 2024 Nov;22(11):3202

Inserts

NbGP41-GFP-AvrXa10-GB1
dCas9-24XGP41
gRNAs targeting FT locus
RFP

Use

Synthetic Biology

Tags

GB1, GFP, and GP41

Expression

Plant

Promoter

Arabidopsis U6, Arabidopsis Ubi10, and Arabidopsi…

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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P-rTetO-EGFP-T2A-RPL22-3xHA

Plasmid

#170326

Purpose

Tetracycline-inducible expression of HA and EGFP tagged RPL22

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pdCas9-GFP

Plasmid

#181906

Purpose

Fluorescently tagged dead Cas9

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

dCas9

Use

CRISPR

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Grin2a KI

Plasmid

#131486

Purpose

Endogenous tagging of GluN2a: N-terminal (amino acid position: A25)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Grin2a Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGFP-hPMLIII

Plasmid

#181903

Purpose

Fluorescently tagged PMLIII

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

hPMLIII (PML Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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R619-M91-303: CMV51p> HCoV-NL63 S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166017

Purpose

mammalian expression of HCoV-NL63 soluble spike trimer protein

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

HCoV-NL63 S (spike)

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Grin2b KI

Plasmid

#131487

Purpose

Endogenous tagging of GluN2b: N-terminal (amino acid position: S34)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Grin2b Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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R619-M90-303: CMV51p> HCoV-229E S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166016

Purpose

mammalian expression of HCoV-229E soluble spike trimer protein

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

HCoV-229E S (spike) (S )

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pNK5664

Plasmid

#219747

Purpose

DVK_AF CIDAR vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of CMV promoter, for mammalian expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pCMV - nnLuz_v4 - tSV40

Use

Luciferase

Expression

Mammalian

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

Plasmid

#167849

Purpose

Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp10, nsp16

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 22, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pSH-EFIRES-P-AtAFB2-mCherry-weak NLS

Plasmid

#129717

Purpose

AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry-weak NLS

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

AFB2 (AFB2 Mustard Weed)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

mCherry-weak NLS

Expression

Mammalian

Mutation

codon optimized

Promoter

EF1a

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.23-5xE-box

Plasmid

#206841

Purpose

The plasmid contains a 312 bp DNA fragment that contained 5 canonical E-boxes (GCCACGTGCA) spaced by 50 nucleotides and cloned into pGL4.23[luc2/minP] (XhoI/HindIII).

Depositor

Tassula Proikas-Cezanne

Article

Sporbeck et al Commun Biol. 2023 Aug 24;6(1):872. doi:

Insert

5x E-box ((GCCACGTGCA)

Use

Luciferase

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pRN120

Plasmid

#160696

Purpose

BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, CmYLCV::STM::35S terminator, Nos::WUS2::PinII terminator

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Inserts

CmYLCV::Luc+::AtHSP
Nos::WUS2::PinII
AtUbi10::STM::35S

Use

Luciferase; Gemini viral replicon, plant developm…

Promoter

AtUbi10, CmYLCV, and Nos

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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