We narrowed to 30,836 results for: Ide

1. pCMV-M2-TLR2

   Plasmid

   #12291

   Depositor

   Bruce Beutler

   Article

   Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   TLR2 (Tlr2 Mouse)
   

   Tags

   FLAG (FLAG-M2)

   Expression

   Mammalian

   Mutation

   M82I and H202Y; signal peptide (aa1-23) replaced …

   Available Since

   Jan. 25, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pAC150-PBLHL-4xHS-EF1a-DEST

   Plasmid

   #48234

   Purpose

   PiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection marker

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Type

   Empty backbone

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. ShuttleNSE

   Plasmid

   #50958

   Purpose

   A backbone for adenovirus creation by the AdEasy method, similar to pShuttle-CMV (Addgene #16403), except it uses a Neuron-specific enolase (NSE) promoter instead of the CMV promoter

   Depositor

   James Bamburg

   Article

   Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

   Type

   Empty backbone

   Use

   Adenoviral

   Expression

   Mammalian

   Promoter

   NSE

   Available Since

   Feb. 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pAAV.hSyn-FLEX.iGABASnFR

   Plasmid

   #112163

   Purpose

   GABA Sensor

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

   Has Service

   AAV1

   Insert

   iGABASnFR
   

   Use

   AAV

   Promoter

   Synapsin

   Available Since

   July 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEMT-PT2A-GFP-Tdtomato-iRFP670

   Plasmid

   #111812

   Purpose

   Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   GFP
   Tdtomato
   iRFP670
   

   Use

   Cloning intermediate

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV mouse Dscam

   Plasmid

   #18737

   Depositor

   Joshua Sanes

   Article

   Yamagata et al Nature. 2008 Jan 24. 451(7177):465-9.

   Insert

   Dscam (Dscam Mouse)
   

   Expression

   Mammalian

   Available Since

   Aug. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

7. CMV-O-GECO1

   Plasmid

   #46025

   Purpose

   Orange intensiometric genetically encoded Ca2+-indicators for optical imaging

   Depositor

   Robert Campbell

   Article

   Wu et al ACS Chem Neurosci. 2013 Mar 19.

   Insert

   O-GECO1
   

   Expression

   Mammalian

   Mutation

   R-GECO1 N45I/A73V/S142P/M146R/M223T/M339L/K378R/E…

   Promoter

   CMV

   Available Since

   July 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSEED-mScarlet3:ALFA

   Plasmid

   #219641

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in mScarlet3:ALFA using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   mScarlet:ALFA SEED Cassette
   

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMD.RVG.CVS24-N2c

   Plasmid

   #19712

   Depositor

   Manfred Schubert

   Article

   Mentis et al J Neurosci Methods. 2006 Oct 30. 157(2):

   Insert

   Rabies virus CVS24-N2c glycoprotein
   

   Expression

   Mammalian

   Available Since

   Nov. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-FLAG-CaMKIIB

    Plasmid

    #186826

    Purpose

    Expresses FLAG-CaMKIIB in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    Camk2b (Camk2b Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEX-A-U6-gRNA

    Plasmid

    #65626

    Purpose

    Empty vector for the expression U6 driven gRNA

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    U6-gRNA
    

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMLM3705

    Plasmid

    #47754

    Purpose

    Expresses mammalian cell codon-optimized dCas9-VP64

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    codon optimized dCas9-VP64
    

    Use

    CRISPR

    Tags

    3x FLAG, NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A and H840A mutations in Cas9 (catalytically i…

    Promoter

    CMV

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiCRISPRv2-scr

    Plasmid

    #169795

    Purpose

    Expresses Cas9 and guide RNA with untarget sequence generated by scramble of the target sequence of LentiCRISPRv2-ACTB-C1..

    Depositor

    Natsuko Chiba

    Article

    Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

    Insert

    Scrambled guide RNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. p2CT-His-MBP-Lsh_C2c2_WT

    Plasmid

    #83487

    Purpose

    Bacterial expression of WT L. shahii C2c2 CRISPR effector

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lsh_C2c2_WT
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAT15489_dABE8e

    Plasmid

    #174127

    Purpose

    plasmid expressing ABE8e with dCas9

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    dABE8e
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Promoter

    EF1-a

    Available Since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3NES-CS(G)-tTA-CS(G)-3NES

    Plasmid

    #137836

    Purpose

    Soluble tTA transcription factor flanked by N and C terminal TEV protease G cleavage sequences and 3 nuclear export sequences

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    3NES-CD(G)-tTA-CS(G)-3NES
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ubiq-Cas9.874W

    Plasmid

    #112686

    Purpose

    Express hSpCas9 under Ubiquitin-63E promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    hSpCas9
    

    Tags

    3x FLAG, EGFP, and NLS

    Expression

    Insect

    Mutation

    Humanized Cas9 bacterial sequence

    Promoter

    Ubiquitin-63E promoter

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDAS12125_PEAR-GFP

    Plasmid

    #177178

    Purpose

    PEAR-GFP target plasmid with split EGFP and disrupted 5' splice site

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMarVF1

    Plasmid

    #99579

    Purpose

    Minitransposon delivery vector pMarVF1 (suicide vector) in V. fischeri ES114

    Depositor

    Mark Mandel

    Article

    Brooks JF et al Proc Natl Acad Sci U S A. 2014 Dec 2;111

    Insert

    V. fischeri mariner transposon (with erythromycin-resistance (ErmR) cassette)
    

    Use

    Suicide vector in v. fischeri es114

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPCK

    Plasmid

    #133128

    Purpose

    a recombinant plasmid, expressing pckA PEP-carboxykinase) gene

    Depositor

    Gregg Beckham

    Article

    Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    PEP-carboxykinase gene
    

    Expression

    Bacterial

    Promoter

    PEP-carboxykinase promoter

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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