We narrowed to 12,610 results for: NSI
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Plasmid#169224PurposeTargeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Puro at the C-terminus of a target locusDepositorInsertDouble SapI flanked mTurquoise2-P2A-Puro
UseTargeting vector backboneAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SARS2-S-FKO (NC-flag)
Plasmid#159363PurposeMammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.DepositorInsertSARS-CoV-2 S protein (S SARS-CoV-2)
TagsFlag and FlagExpressionMammalianMutationFurin-cleavage site mutationPromoterCMVAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV2 TEV-E
Plasmid#154418PurposeGateway-compatible Entry vectorDepositorInsertE (E SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of e…MutationMany synonymous changes due to codon optimizationAvailable SinceJuly 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAGGS-SARS2-S-G614 (NC-flag)
Plasmid#156419PurposeMammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.DepositorInsertSARS-CoV-2 S protein (S SARS CoV2)
TagsFlagExpressionMammalianMutationD614GPromoterCMVAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF-D594N
Plasmid#131713PurposeMammalian Expression of Flag tagged BRAFDepositorAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Eomes
Plasmid#128825Purposedox-dependent expression of mouse EomesDepositorAvailable SinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_CRFmut
Plasmid#208663PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in a cre-dependent mannerDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
UseAAVPromoterEFSAvailable SinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-HOXD13-IDR-mCherry-CRY2-NLS
Plasmid#145260PurposeMammalian Expression of Nuclear Opto HOXD13 IDR with SV40 NLSDepositorInsertHOXD13wt-IDR (HOXA13 Human)
ExpressionMammalianAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF692
Plasmid#88701PurposeDonor Vector containing ZNF692 transcription factor, part of the Human TFome CollectionDepositorInsertZNF692 (ZNF692 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV2 TEV-M
Plasmid#154419PurposeGateway-compatible Entry vectorDepositorInsertM (M SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of m…MutationMany synonymous changes due to codon optimizationAvailable SinceJuly 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223-ZNF770
Plasmid#88562PurposeDonor Vector containing ZNF770 transcription factor, part of the Human TFome CollectionDepositorInsertZNF770 (ZNF770 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
Myc-Nix-delta TM
Plasmid#100755PurposeMammalian expression of Nix dominant negativeDepositorAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dCas9_sgRNA NC
Plasmid#176259PurposepNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI andDepositorInsertdead spCas9
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseMutationH840A and D10A mutations on spCas9 to inactivate …Available SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF768
Plasmid#88524PurposeDonor Vector containing ZNF768 transcription factor, part of the Human TFome CollectionDepositorInsertZNF768 (ZNF768 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon/Von GCaMP 6m
Plasmid#137165PurposeIntersectional viral expression of GCaMP6M in cells expressing Cre AND Flp AND VcreDepositorInsert3x-GCaMP6M
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationRemoved RSET tagPromoterEf1aAvailable SinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon-bREACHes-EYFP
Plasmid#137145PurposeIntersectional viral expression of bREACHes-EYFP in cells expressing both Cre AND FlpDepositorInsertCon/Fon-bREACHes-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationn/aPromoternEFAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-HOXD13(+10A)-IDR-mCherry
Plasmid#145282PurposeBacterial Expression of 6xHis HOXD13 (+10A) IDR mCherry Fusion ProteinDepositorAvailable SinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBT270_(pCA-G-intron(Neo)-tTA2-iiTRE-tdT3Mycii)
Plasmid#36881DepositorInsertsGFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator
Tags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available SinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A STINGHAQ V155M
Plasmid#102592PurposeLentivector to express STING V155M HAQ and mTagBFPDepositorInsertSTING (STING1 Human)
UseLentiviralExpressionMammalianMutationV155M R71H-G230A-R293QPromoterSFFVAvailable SinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Myc-Nix-MaoB
Plasmid#100757PurposeMammalian expression of mitochondria targetted NixDepositorInsertNix-MaoB (BNIP3L Human)
TagsMycExpressionMammalianMutationTM domain replaced by MaoBPromoterCMVAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only