We narrowed to 166,303 results for: addgene

1. MSP469

   Plasmid

   #65771

   Purpose

   Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135V, R1335Q, and T1337R mutations in Cas9

   Promoter

   CMV & T7

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAGGS-EGFP-NLS-3XFLAG-V5

   Plasmid

   #196090

   Purpose

   Expresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Insert

   GFP
   

   Tags

   C-terminal 3xFLAG and C-terminal V5

   Expression

   Bacterial and Mammalian

   Promoter

   CAGGs

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEN_TT 3xFLAG ID2

   Plasmid

   #98390

   Purpose

   Gateway entry vector for inducible expression of human ID2 with N-terminal 3xFLAG tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   ID2 (ID2 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   3xFLAG

   Promoter

   TRE tight promoter

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. 426Gal-FUS-YFP

   Plasmid

   #29592

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Tags

   YFP

   Expression

   Yeast

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-BAF

   Plasmid

   #227442

   Purpose

   Mammalian expression of GFP-BAF

   Depositor

   David Pellman

   Article

   Umbreit et al Science. 2020 Apr 17;368(6488):eaba0712.

   Insert

   Barrier-to-autointegration factor (BANF1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6 SgRNA RBM20

   Plasmid

   #162735

   Purpose

   Mammalian expression

   Depositor

   Amit Choudhary

   Article

   Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

   Insert

   Spacer sequence for RBM20
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1-TRC.mKO2

   Plasmid

   #85208

   Purpose

   Backbone for lentiviral gene silencing with monomeric Kusabira-Orange2 expression

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWPXL-Sox4

   Plasmid

   #36984

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   Sox4 (SOX4 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEN244 - CTCF-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

   Plasmid

   #92140

   Purpose

   Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using BLASTICIDIN selection. Auxin-inducible degron system.

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   AID[71-114]-eGFP
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-C-moxGFP-Puro-H2BC11

    Plasmid

    #207758

    Purpose

    Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 17, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pyc126m

    Plasmid

    #158777

    Purpose

    ribosome-tagged GCaMP for soma localization

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    GCaMP6m-RPL10a
    

    Use

    AAV

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-EGFP barcode-1-SV40 polyA

    Plasmid

    #190865

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode1
    

    Use

    AAV

    Mutation

    WT

    Available Since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pyc111s

    Plasmid

    #158781

    Purpose

    ribosome-tagged, Cre-dependent GCaMP for soma localization

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    GCaMP6s-RPL10a
    

    Use

    AAV

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. EGFP-P2A_BAF_L58R

    Plasmid

    #101775

    Purpose

    Expresses mutant BAF (L58R) in human cells (with EGFP produced from the same transcript as expression control)

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    BAF (BANF1) (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP-P2A

    Expression

    Mammalian

    Mutation

    L58R mutation

    Promoter

    EF1a

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. MAC_C_AIFM1

    Plasmid

    #111659

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    AIFM1 (AIFM1 Human)
    

    Expression

    Mammalian

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAVS1-NDi-CRISPRd (ABI-dCas9-mCherry)

    Plasmid

    #216531

    Purpose

    Targeting vector for CRISPRd generation

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    ABI-dCas9-mCherry
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSP680

    Plasmid

    #65772

    Purpose

    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135E, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDH_CMV_dimer_mKate2

    Plasmid

    #167333

    Purpose

    Expresses mKate2 fused to TFAM no-dimerization mutant in mammalian cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    TFAM (TFAM Human)
    

    Use

    Lentiviral

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Changed K95A, Y99F, E106A, E112A and R116A

    Promoter

    CMV

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDH_CMV_HMGA_mKate2

    Plasmid

    #167334

    Purpose

    Expresses mKate2 fused to TFAM HMGA mutant in mammalian cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    TFAM (TFAM Human)
    

    Use

    Lentiviral

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Truncation mutant containing MTS (1-42) and HMGA …

    Promoter

    CMV

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330-PITCh-CLTC

    Plasmid

    #227312

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CLTC for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of CLTC (CLTC Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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