We narrowed to 16,015 results for: grn

1. pX330-U6-L101_guide-CBh-hSpCas9

   Plasmid

   #188547

   Purpose

   Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid L101

   Depositor

   Darrell Kotton

   Article

   Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   T2A-EGFP

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p204-U6-Switch-ON

   Plasmid

   #217883

   Purpose

   Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

   Depositor

   Ulrich Elling

   Article

   Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV.5’eGFP/sgLMNA

   Plasmid

   #170546

   Purpose

   Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP without homology arms

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Insert

   sgRNA targeting 5'- end of LMNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV.5’eGFP/sgVIM

   Plasmid

   #170548

   Purpose

   Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP without homology arms

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Insert

   sgRNA targeting 5'- end of VIM
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-Cas9-NS-A

   Plasmid

   #207823

   Purpose

   Dual expression of Cas9 and non-specific sgRNA

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   non-specific sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   EF-1a; U6

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-Cas9-NS-B

   Plasmid

   #207824

   Purpose

   Dual expression of Cas9 and non-specific sgRNA

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   non-specific sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   EF-1a; U6

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pML45

   Plasmid

   #206995

   Purpose

   Contains gRNA targeting mouse Ptbp1 gene and Cas9 ORF.

   Depositor

   Eugene Makeyev

   Article

   Kainov et al STAR Protoc. 2023 Oct 18;4(4):102644. do

   Insert

   Ptbp1 gRNA (Ptbp1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRANS-Pho2-1/2-2-Csy4

   Plasmid

   #161763

   Purpose

   pTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x Csy4-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)

   Depositor

   Shaun Curtin

   Article

   Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

   Insert

   Cas9 and gRNAs
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CmYLCV Promoter

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRANS-Pho2-1/2-2-tRNA

   Plasmid

   #161762

   Purpose

   pTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x tRNA-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)

   Depositor

   Shaun Curtin

   Article

   Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

   Insert

   Cas9 and gRNAs
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CmYLCV Promoter

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSCL.178

    Plasmid

    #184999

    Purpose

    Express -Eco1 FANCF editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: FANCF targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    FANCF donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSCL.176

    Plasmid

    #184997

    Purpose

    Express -Eco1 RNF2 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: RNF2 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    RNF2 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSCL.179

    Plasmid

    #185000

    Purpose

    Express -Eco1 HEK4 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: HEK4 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    HEK4 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLiP-G1G2

    Plasmid

    #188965

    Purpose

    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

    Depositor

    Seung-Goo Lee

    Article

    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

    Inserts

    dCas9
    sgRNA 1: atcggtcgcattgttttccactagg, sgRNA 2: gttagacgctgattacatggactagg
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaBAD

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTW045

    Plasmid

    #185690

    Purpose

    T-DNA for creating transgenic plants expressing Cas9 and Drm1b gRNAs

    Depositor

    Feng Zhang

    Article

    Weiss et al Plant J. 2020 Nov;104(3):828-838. doi: 1

    Insert

    Cas9, Drm1b gRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTW037

    Plasmid

    #185688

    Purpose

    T-DNA for creating transgenic plants expressing Cas9 and Drm1b gRNAs

    Depositor

    Feng Zhang

    Article

    Weiss et al Plant J. 2020 Nov;104(3):828-838. doi: 1

    Insert

    Cas9, Drm1b gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSC51

    Plasmid

    #104825

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Glyma.12g075700, Glyma.11g145900 (Drb2ab). Also expresses Cas9 from rolD promoter from Gmubi promoter

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Insert

    Glyma.12g075700, Glyma.11g145900
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pROS13_IDH2 #7

    Plasmid

    #166102

    Purpose

    This plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.

    Depositor

    Matthias Heinemann

    Article

    Chen et al bioRxiv

    Insert

    IDH2 sgRNA #7 (IDH2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pROS13_IDH1 #4

    Plasmid

    #166101

    Purpose

    This plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.

    Depositor

    Matthias Heinemann

    Article

    Chen et al bioRxiv

    Insert

    IDH1 sgRNA #4 (IDH1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO-Cre sgUroD v4

    Plasmid

    #158036

    Purpose

    lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse UroD

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    UroD sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pLKO-Cre sgItgb5 v3

    Plasmid

    #158038

    Purpose

    lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Itgb5

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    Itgb5 sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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