We narrowed to 9,345 results for: yeast expression

1. pAWH-IRP

   Plasmid

   #133896

   Purpose

   Positive control when used with pMS22H-IRE. Expression of Gal4AD-IRP hybrid protein. Homology regions for recombination with pMS22H

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Insert

   IRP
   

   Expression

   Yeast

   Promoter

   T7

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS-ATG1-ATG7 3'UTR(406)

   Plasmid

   #122070

   Purpose

   Expressing ATG1 with ATG7 3'UTR

   Depositor

   Daniel Klionsky

   Article

   Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

   Insert

   serine/threonine protein kinase ATG1 (ATG1 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   The native 3'UTR was replaced by ATG7 3'…

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCu-GFP-Atg17(416)

   Plasmid

   #121408

   Purpose

   Expressing GFP-Atg17

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   CUP1p-GFP-ATG17 ORF-CYC1 terminator
   

   Expression

   Yeast

   Promoter

   CUP1 promoter

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCu-Atg17(416) (F317D)

   Plasmid

   #121406

   Purpose

   Expressing Atg17

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   CUP1p-ATG17 ORF-CYC1 terminator
   

   Expression

   Yeast

   Mutation

   Phenylalanine 317 to Aspartic acid

   Promoter

   CUP1 promoter

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCu-Atg17(416) (I325D)

   Plasmid

   #121407

   Purpose

   Expressing Atg17

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   CUP1p-ATG17 ORF-CYC1 terminator
   

   Expression

   Yeast

   Mutation

   Isoleucine 325 to Aspartic acid

   Promoter

   CUP1 promoter

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. p415TEF-NYFP

   Plasmid

   #99555

   Purpose

   Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

   Depositor

   Liming Li

   Article

   Hines et al PLoS Genet. 2011 Feb;7(2):e1001309. doi:

   Insert

   Swi1 N-rich region (AA1-327) (SWI1 Budding Yeast)
   

   Tags

   YFP

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   Aug. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWHI2-HYG

   Plasmid

   #80589

   Purpose

   CEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoter

   Depositor

   John McCusker

   Article

   Vijayraghavan et al Genetics. 2018 Nov 29. pii: genetics.118

   Insert

   WHI2 (WHI2 Budding Yeast)
   

   Expression

   Yeast

   Promoter

   WHI2 native promoter

   Available Since

   July 28, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pYES2-gRNA-hyg-MCS

   Plasmid

   #107734

   Purpose

   E. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.

   Depositor

   Sheng Yang

   Article

   Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

   Insert

   X-3 site gRNA expression cassettes
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   SNR52p

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. TDH3pro-rtTA-tetO7pro-T7pol-pRS413

   Plasmid

   #239293

   Purpose

   Expresses the reverse tetracycline activator (rtTA) and T7 polymerase; Plasmid #1 of the tet-on overexpression system

   Depositor

   Anita Hopper

   Article

   Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

   Inserts

   T7 polymerase with NLS and P278L
   reverse tetracycline transactivator (rtTA)
   

   Expression

   Yeast

   Mutation

   Contains M2-SE-G72P mutations, described in Addge…

   Promoter

   TDH3 and tetracycline-responsive element (TRE) an…

   Available Since

   June 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYTRW10K_0x5

    Plasmid

    #177278

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

    Depositor

    Karl-Erich Jaeger

    Article

    Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Yeast expression, tn5 genomic …

    Expression

    Bacterial

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSCL475

    Plasmid

    #219503

    Purpose

    Express Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in trans

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

    Insert

    Ade2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
    

    Expression

    Yeast

    Promoter

    GAL7

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pITESceIcmd

    Plasmid

    #218288

    Purpose

    Introduction of a cyanamide-inducible I-SceI expression cassette, use in combination with pITEdv1

    Depositor

    Claudia Vickers

    Article

    McDonnell et al bioRxiv 2024.03.26.586389

    Insert

    HO(-253, -1)-tSynth3

    Expression

    Yeast

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-ATG8(416)/GFP-AUT7(416)

    Plasmid

    #49425

    Purpose

    Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

    Depositor

    Daniel Klionsky

    Article

    Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

    Insert

    autophagy-related 8 (ATG8 Budding Yeast)
    

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Promoter

    ATG8

    Available Since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDIV515

    Plasmid

    #177703

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    tRNA-sgRNA-tRNA
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Promoter

    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDIV537

    Plasmid

    #177704

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    tRNA-sgRNA-tRNA
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Promoter

    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDIV494

    Plasmid

    #177701

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    sgRNA Targeting ADE2 locus in D.hansenii: AGCTAAGCAGATTAATGCAT
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Promoter

    RNR2p (Debaryomyces hansenii ), SNR52p (Candida s…

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas3-APOBEC1

    Plasmid

    #229536

    Purpose

    pMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    rAPOBEC1
    Cas3
    Uracil glycosylase inhibitor
    

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas3-CDA1

    Plasmid

    #229534

    Purpose

    pMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    Cas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDIV495

    Plasmid

    #177702

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    sgRNA Targeting ADE2 locus in D.hansenii: AGCTAaGCAGATTAAtGCAT
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Promoter

    RNR2p (Debaryomyces hansenii ), SRC1p (Debaryomyc…

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pH3mCHSH2

    Plasmid

    #101053

    Purpose

    This is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.

    Depositor

    Jennifer Lodge

    Article

    Upadhya et al Fungal Genet Biol. 2017 Sep 1. pii: S108

    Insert

    Cryptococcus mCherry expression plasmid
    

    Expression

    Yeast

    Promoter

    Cryptococcus Histone3 promoter

    Available Since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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