Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 12,610 results for: NSI

Showing: 8641 - 8660 of 12610 results

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pAAV-nEF-Coff/Fon-bREACHes-EYFP

Plasmid

#137147

Purpose

Intersectional viral expression of bREACHes-EYFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Insert

Coff/Fon-bREACHes-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF545

Plasmid

#88479

Purpose

Donor Vector containing ZNF545 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF545 (ZFP82 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF132

Plasmid

#88563

Purpose

Donor Vector containing ZNF132 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF132 (ZNF132 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 SARS-CoV-2 S-frag3_nostop

Plasmid

#153175

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag3

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pHR-HOXD13(+9A)-IDR-mCherry-CRY2-NLS

Plasmid

#145263

Purpose

Mammalian Expression of Nuclear Opto HOXD13(+9A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(+9A)-IDR (HOXD13 Human)

Expression

Mammalian

Mutation

A-repeat expanded

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221 SARS-CoV-2 S-frag4_nostop

Plasmid

#153176

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag4

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223-ZNF76

Plasmid

#88678

Purpose

Donor Vector containing ZNF76 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF76 (ZNF76 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF32

Plasmid

#88309

Purpose

Donor Vector containing ZNF32 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF32 (ZNF32 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 SARS-CoV-2 S-frag1_nostop

Plasmid

#153173

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag1

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-ZNF716

Plasmid

#101481

Purpose

Donor Vector containing ZNF716 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF716 (ZNF716 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF680

Plasmid

#88455

Purpose

Donor Vector containing ZNF680 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF680 (ZNF680 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-29Q

Plasmid

#84350

Purpose

Expression of the human Huntingtin Exon1 protein containing 29Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon 1 (HTT Human)

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-D594V

Plasmid

#131715

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D594V

Promoter

CMV

Available Since

May 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF165

Plasmid

#88444

Purpose

Donor Vector containing ZNF165 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF165 (ZNF165 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pNOC_dfnCas12a_crRNA NC

Plasmid

#176256

Purpose

pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and spacer sequence is replaced by the type IIS restriction site for endonuclease BaeI that can be

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Mutation

D917A and E1006A mutations to inactivate the endo…

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF467

Plasmid

#88643

Purpose

Donor Vector containing ZNF467 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF467 (ZNF467 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLL5.0 ShCavin1-EGFP

Plasmid

#187253

Purpose

Lentiviral expression of shRNA targeting Cavin1

Depositor

Alpha Yap

Article

Teo et al Dev Cell. 2020 Jul 6;54(1):75-91.e7. doi

Insert

ShRNA for Cavin1 (Cavin1 Mouse)

Use

Lentiviral

Tags

EGFP

Promoter

U6

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-15Q

Plasmid

#84348

Purpose

Expression of the human Huntingtin Exon1 protein containing 15Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon1 (HTT Human)

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-V600K

Plasmid

#131720

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

V600K

Promoter

CMV

Available Since

Dec. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF674

Plasmid

#88785

Purpose

Donor Vector containing ZNF674 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF674 (ZNF674 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 8641 - 8660 of 12610 results

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