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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 171,419 results for: addgene

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Showing: 8641 - 8660 of 171419 results

  3. pBAD-bHs

    Plasmid
    #134259
    Purpose
    For L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tag
    Depositor
    Edwin Manuel
    Article
    Ebelt et al Mol Cancer Ther. 2019 Nov 6. pii: 1535-7
    Insert
    Hyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    codon optimized for Salmonella typhimurium
    Promoter
    AraC
    Available Since
    Nov. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    134259-plasmid-map-sequence-id-264461

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  8. MSP2133

    Plasmid
    #72249
    Purpose
    Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    Y450A, N497A, R661A, Q695A, and Q926A
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72249-plasmid-map-sequence-id-131869

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  9. MSP2135

    Plasmid
    #72248
    Purpose
    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, and D1135E
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72248-plasmid-map-sequence-id-131868

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  14. qTAG-C-Solo-mStayGold-H2BC11

    Plasmid
    #227330
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a mStayGold Tag (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227330-plasmid-map-sequence-id-452124

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  17. pRosa26-GT

    Plasmid
    #40025
    Depositor
    Liqun Luo
    Article
    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma
    Inserts
    GFP
    beta-globin intron
    Neo
    tdT-3Myc
    diphteria toxin A
    Tags
    3 Myc tags
    Expression
    Mammalian
    Mutation
    deleted nucleotides after nucleotide 274, inserti…
    Promoter
    CAG (chicken beta globin promoter and CMV enhance…
    Available Since
    Oct. 22, 2012
    Availability
    Academic Institutions and Nonprofits only
    40025-plasmid-map-sequence-id-210933

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  19. pCALNL-Rheb(S16H)

    Plasmid
    #194204
    Purpose
    Cre/LoxP-dependent expression of a constitutively active form of Rheb (S16H). Expression of Cre excises a STOP codon before Rheb(S16H), facilitating expression of a constitutively active form of Rheb.
    Depositor
    Angelique Bordey
    Article
    Lin et al Proc Natl Acad Sci U S A. 2016 Oct 4;113
    Insert
    Ras Homolog Enriched Protein in Brain (S16H) (RHEB Human)
    Use
    Cre/Lox
    Expression
    Mammalian
    Mutation
    S16H
    Available Since
    Jan. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    194204-plasmid-map-sequence-id-382462

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Showing: 8641 - 8660 of 171419 results