We narrowed to 166,303 results for: addgene

1. pCALNL-Rheb(S16H)

   Plasmid

   #194204

   Purpose

   Cre/LoxP-dependent expression of a constitutively active form of Rheb (S16H). Expression of Cre excises a STOP codon before Rheb(S16H), facilitating expression of a constitutively active form of Rheb.

   Depositor

   Angelique Bordey

   Article

   Lin et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

   Insert

   Ras Homolog Enriched Protein in Brain (S16H) (RHEB Human)
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Mutation

   S16H

   Available Since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TET-pLKO.1 PURO shSMAD4 #1

   Plasmid

   #83089

   Purpose

   Lentiviral shRNA vector for inducible knockdown of human SMAD4 (cross reacts with mouse Smad4)

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   shSMAD4 (SMAD4, Smad4 Mouse, Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

3. pAS_4xMmaPylT(UCA)_EF1_MmaPylRS

   Plasmid

   #174899

   Purpose

   Mma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CDK1as_T2A_Zeo

   Plasmid

   #118596

   Purpose

   One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.

   Depositor

   William Earnshaw

   Article

   Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

   Insert

   CDK1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRosa26-GT

   Plasmid

   #40025

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   beta-globin intron
   Neo
   tdT-3Myc
   diphteria toxin A
   

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, inserti…

   Promoter

   CAG (chicken beta globin promoter and CMV enhance…

   Available Since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. SNAP-BAF

   Plasmid

   #227443

   Purpose

   Mammalian expression of SNAP-BAF

   Depositor

   David Pellman

   Article

   Umbreit et al Science. 2020 Apr 17;368(6488):eaba0712.

   Insert

   Barrier-to-autointegration factor (BANF1 Human)
   

   Use

   Lentiviral

   Tags

   SNAP

   Expression

   Mammalian

   Available Since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC2-dual-dCas9VP48-sgExpression

   Plasmid

   #48236

   Purpose

   Dual expression construct expressing both dCas9VP48 and sgRNA from separate promoters

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9VP48
   

   Use

   CRISPR

   Tags

   HA-tag and VP48

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive)

   Available Since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-PITCh-PEX3

   Plasmid

   #227303

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PEX3 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of PEX3 (PEX3 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-RAB7A

   Plasmid

   #227297

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of RAB7A for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of RAB7A (RAB7A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-C-moxGFP-Zeo-H2BC11

    Plasmid

    #207759

    Purpose

    Donor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 17, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. 416Gal-FUS-YFP

    Plasmid

    #29593

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Tags

    YFP

    Expression

    Yeast

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pRMC2-tat:msfGFP

    Plasmid

    #194915

    Purpose

    Tetracycline inducible expression of msfGFP fused to Tat signal peptide in Staphylococci

    Depositor

    Rikke Meyer

    Article

    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

    Insert

    Shine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
    

    Use

    Tetracycline-inducible expression vector for stap…

    Expression

    Bacterial

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. LLP786_SpyCatcher-EZH2-FL-CO

    Plasmid

    #211779

    Purpose

    SpyTag counterpart binding domain, SpyCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-KRAB
    

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect KRAB e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pATZ-HA-GFP11

    Plasmid

    #177720

    Purpose

    ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to an HA tag and the eleventh β-strand of GFP

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    alpha-1-antitrypsin (SERPINA1 Human)
    

    Tags

    GFP11 and HA

    Expression

    Bacterial and Mammalian

    Mutation

    E342K (ATZ mutant)

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAD-bHs

    Plasmid

    #134259

    Purpose

    For L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tag

    Depositor

    Edwin Manuel

    Article

    Ebelt et al Mol Cancer Ther. 2019 Nov 6. pii: 1535-7

    Insert

    Hyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    codon optimized for Salmonella typhimurium

    Promoter

    AraC

    Available Since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBabe human FOXC2

    Plasmid

    #15535

    Depositor

    Bob Weinberg

    Article

    Mani et al Proc Natl Acad Sci U S A. 2007 Jun 12. 1

    Insert

    FOXC2 (FOXC2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

17. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

    Plasmid

    #124537

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro
    

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41 C55G

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pyc111f

    Plasmid

    #158780

    Purpose

    ribosome-tagged, Cre-dependent GCaMP for soma localization

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    GCaMP6f-RPL10a
    

    Use

    AAV

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSL1180-HR-PUbEBFP2-NLS

    Plasmid

    #176682

    Purpose

    Expression of nuclear localized EBFP2 under Ae. aegypti Polyubiquitin promoter (AAEL003888), EBFP2 version of pSL1180-HR-PUbECFP (Addgene #47917)

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Insert

    EBFP2
    

    Expression

    Insect

    Promoter

    Ae. aegypti poly-ubiquitin (AAEL003888)

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. S1-Fc

    Plasmid

    #164220

    Purpose

    Expresses soluble, dimeric Fc-His fusion protein for the original SARS-CoV2 Spike S1 region, along with dTomato reporter

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

    Insert

    Spike S1 domain (S SARS-CoV)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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