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Plasmid#47941PurposeCan be used to generate synthetic guide in vitro from T7 promotorDepositorPublicationInsertsynthetic guide RNA
UseCRISPRExpressionWormAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pIDS-LSD1fl
Plasmid#109157PurposeEncodes human full-length LSD1 to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length LSD1, sequence-optimized for insect cells (KDM1A Human)
ExpressionInsectPromoterp10Available sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO.1-TetON-Puro-HIF1a 3809
Plasmid#118705PurposeLentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)DepositorInsertshRNA against human HIF1a
UseLentiviralAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-hygro-STING HAQ
Plasmid#102600PurposeExpress STING HAQ for IFN-beta Luciferase reporter assay.DepositorAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV_BiPVe3_ChR2_mCherry
Plasmid#213941PurposeAAV construct expressing mCherry-tagged channelrhodopsin-2 driven by PV+ basket cell-targeting enhancer. Alias: AAV_WDP0003_ChR2_mCherryDepositorHas serviceAAV PHP.eBInsertChannelrhodopsin 2-mCherry
UseAAVAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJL1-mCherry
Plasmid#102629PurposeIn vitro expression of mCherry from the T7 promoterDepositorInsertmCherry
ExpressionBacterialPromoterT7Available sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS1096 Dual-sgRNA.Design 1
Plasmid#159538PurposeDelivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vectorDepositorInsertNme2Cas9 nuclease with two guide RNA cassettes with promoters
UseAAV, CRISPR, and Mouse TargetingTagsNLSExpressionMammalianPromoterU1aAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
LiOn-CMV∞Cre
Plasmid#154018PurposeVector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing a functional Cre recombinase from a CMV promoter upon action of the piggyBac transposaseDepositorInsertCre-FLAG
ExpressionMammalianPromoterCMVAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A STING V155M
Plasmid#102591PurposeLentivector to express STING V155M and mTagBFPDepositorAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUDC175
Plasmid#103019PurposeS. cerevisae centromic plasmid harboring Fncpf1 under control of TEF1 promoterDepositorInsertFncpf1
Tags3HA and NLSExpressionYeastMutationhuman codon optimizedPromoterTEF1Available sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMycVec2
Plasmid#32361DepositorPublicationInsertpJAZ38 ori
ExpressionBacterialAvailable sinceNov. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
LiOn-CAG∞GFP
Plasmid#154015PurposeVector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein EGFP from a CAG promoter upon action of the piggyBac transposaseDepositorInsertEGFP
ExpressionMammalianPromoterCAGAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAWG-dCas9-VPR
Plasmid#63802PurposeSP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; drosophila vectorDepositorInsertSP-dCas9-VPR
UseCRISPR and Synthetic BiologyTagsVPR (VP64-p65-Rta)ExpressionInsectPromoteract5cAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1(+) NAPstar3b
Plasmid#232736PurposeExpresses NADPH/NADP+ biosensor NAPstar3b in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNAPstar3b
ExpressionMammalianAvailable sinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
OpenCRISPR-1 sgRNA-12nt stem
Plasmid#221567PurposeExpress OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOpenCRISPR-1 sgRNA 12nt stem loop
ExpressionMammalianPromoterU6Available sinceAug. 28, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
PE7_AAVS1_knockin_HDR_donor
Plasmid#223001PurposeHDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_armDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPE7
UseHdr donorTagsSV40 bpNLS and c-Myc NLSExpressionMammalianPromoterEF1αAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (AAV1)
Viral prep#125712-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (#125712). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE plasmid DNA. CaMKIIa-driven expression of the optimized mosquito OPN3 rhodopsin in-frame with mScarlet. These AAV preparations are suitable purity for injection into animals.DepositorPromotershort version of αCamKinase II promoter (0.4kb)TagsmScarletAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX307-CRBN-3F-BirA
Plasmid#220146PurposeExpresses CRBN-BirA for CRBN substrate identification by E-STUBDepositorAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pG3H-PE3max-attR1R2
Plasmid#213049PurposeDestination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sitesDepositorInsertsCas9 nickase
M-MLV Reverse Transcriptase
UseNote: this plasmid needs helper pvs1-vir2 for rep…ExpressionPlantMutationH840A, R240K, N413K,Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mito-B
Plasmid#209864PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVSDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertddGFP B
TagsTargeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…ExpressionMammalianPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only