We narrowed to 17,096 results for: Por

1. PB-tetO-NGN2

   Plasmid

   #197090

   Purpose

   PiggyBac plasmid with tet-inducible expression of transcription factors for cortical neuron differentiation

   Depositor

   Brian Wainger

   Article

   Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

   Insert

   NGN2 (NEUROG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. mApple-Sec61b-C1

   Plasmid

   #90993

   Purpose

   Expresses mApple-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

   Insert

   mApple-Sec61b (SEC61B Human, Synthetic)
   

   Use

   Tags

   mApple (red fluorescence)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1-mTagBFP2-rMPC1 shRNA

   Plasmid

   #229016

   Purpose

   Expression of an shRNA construct that knocks down rat MPC1. This plasmid also encodes a blue fluorescent protein tag to verify transfection

   Depositor

   Ghazaleh Ashrafi

   Article

   Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

   Insert

   MPC1 (Mpc1 Rat)
   

   Use

   Lentiviral

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-ERBB4-V5/HIS

   Plasmid

   #201105

   Purpose

   expression of human ERBB4 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human ERBB4 receptor tyrosine kinase, full length, wildtype (ERBB4 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-AXL-V5/HIS

   Plasmid

   #200117

   Purpose

   Expression of human AXL receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human AXL receptor tyrosine kinase (AXL Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-HA-Ubiquitin-K11

   Plasmid

   #121152

   Purpose

   Mammalian expression of HA tagged ubiquitin with K11 only, other lysines mutated to arginines

   Depositor

   Josef Kittler

   Article

   Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All Ks apart from K11 mutated to Rs

   Promoter

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-mt-mKeima-P2A-FRB-Fis1

   Plasmid

   #135295

   Purpose

   stably express both mKeima and FRB-Fis1 for CID induced mitophagy in mammalian cells

   Depositor

   Richard Youle

   Article

   Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

   Insert

   mt-mKeima-P2A-FRB-Fis1 (FIS1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   mt-mKeima is fused with FRB-Fis1 via P2A seqeunce

   Promoter

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221_SLC5A4

   Plasmid

   #132170

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC5A4 (SLC5A4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pDONR221_SLC5A9

   Plasmid

   #132230

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC5A9 (SLC5A9 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid

    #182287

    Purpose

    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    

    Use

    Tags

    nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Y306A/Y384F (AF) double mutant of Methanosarcina …

    Promoter

    CMV and U6

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-PDGFRA-V5/HIS

    Plasmid

    #234754

    Purpose

    Expression of human PDGFRA receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    PDGFRA receptor tyrosine kinase, full length, wildtype (PDGFRA Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETDuet-1 TIM9,10

    Plasmid

    #170280

    Purpose

    Expresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

    Depositor

    Paul Schanda

    Article

    Weinhaupl et al Cell. 2018 Nov 15;175(5):1365-1379.e25.

    Insert

    mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag) (TIM9, TIM10 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmRFP-N1 human cofilin R21Q

    Plasmid

    #51279

    Purpose

    expresses human cofilin R21Q mutant fused to mRFP in mammalian cells

    Depositor

    James Bamburg

    Article

    Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

    Insert

    cofilin 1 (CFL1 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    R21Q, non-rod-forming mutation that can be used t…

    Promoter

    CMV

    Available Since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pMRXIP-GFP-TRPML1(PHD)

    Plasmid

    #221752

    Purpose

    Stable expression of GFP-TRPML1(PHD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    TRPML1 (MCOLN1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    tandem TRPML1(PHD) (amino acids 1–69)

    Promoter

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRE3G-EBF1

    Plasmid

    #96965

    Purpose

    Dox-inducible expression of Ebf1 in mammalian cells

    Depositor

    Elena Cattaneo

    Article

    Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Insert

    Ebf1 (Ebf1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. MYH11-NanoLuc-2A-tdTomato

    Plasmid

    #132569

    Purpose

    Donor vector to knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele

    Depositor

    James Thomson

    Article

    Zhang et al Stem Cell Reports. 2019 Jun 11;12(6):126

    Insert

    Human MYH11 knockin homology arms (MYH11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3 IRF7(5’UTR)-FF

    Plasmid

    #85490

    Purpose

    Firefly luciferase under the control of IRF7 5'UTR

    Depositor

    Ivan Topisirovic

    Article

    Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

    Insert

    5'UTR of IRF7 (Irf7 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221_SLC16A11

    Plasmid

    #131939

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC16A11 (SLC16A11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 17, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pDONR221_SLC38A7

    Plasmid

    #132094

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC38A7 (SLC38A7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pAAVS1P-iCAG.copGFP

    Plasmid

    #66577

    Purpose

    AAVS1 safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-AAVS1 TALENs

    Depositor

    Jizhong Zou

    Article

    Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

    Insert

    copGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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