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pDEST40-ARF6-V5-His6

Plasmid

#67436

Purpose

Mammalian expression of hArf6 with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF6 (ARF6 Human)

Tags

His and V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pAR-9.47_VP1,3

Plasmid

#74240

Purpose

Original plasmid described in Choudhury et al. 2015 Mol Ther. for production of AAV-AS vectors. Encodes VP1 and VP3 of AAV9.47

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV9.47 VP1
AAV9.47 VP3

Use

AAV

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pONSY-CoNMM:mCherry

Plasmid

#111878

Purpose

This plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.

Depositor

Inaki Ruiz-Trillo

Article

Parra-Acero et al Development. 2018 May 11. pii: dev.16210

Insert

Capsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry

Use

Capsaspora owczarzaki

Tags

mCherry

Promoter

Elongation Factor 1 alpha (EF1a) from Capsaspora

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PA-mCit-GW

Plasmid

#113448

Purpose

ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Gateway shuttle vector

Tags

ProteinA-mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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R

Plasmid

#167170

Purpose

Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant in Sf9 cells

Depositor

Susanne Dobler

Article

Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

Inserts

ATP1A1-R
ATP1B1

Expression

Bacterial and Insect

Promoter

P10 and PH

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pEMS1996

Plasmid

#49126

Purpose

AAV plasmid with UGT8 (Ple267) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple267-iCre

Use

AAV

Expression

Mammalian

Promoter

UGT8

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pET-DEST42-ARL8B-V5-His6

Plasmid

#67425

Purpose

Expression of hArl8b with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8B (ARL8B Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pMC060-HisMS2_PLP_Env_pac

Plasmid

#155040

Purpose

Generation of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Envelope Gene

Depositor

Paul Freemont

Article

Crone et al Nat Commun. 2020 Sep 8;11(1):4464. doi:

Inserts

Maturation Protein
Coat Protein Dimer
Envelope Gene

Expression

Bacterial

Mutation

Remove TypeIIs Restriction Sites

Promoter

T7

Available Since

Sept. 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pET-DEST42-ARF1-V5-His6

Plasmid

#67411

Purpose

Expression of hArf1 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF1 (ARF1 Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
YFP-Rpp40-C1

Plasmid

#134549

Purpose

Expresses YFP-Rpp40 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Rpp40 (RPP40 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARL14-GFP

Plasmid

#67406

Purpose

Mammalian expression of hArl14 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL14 (ARL14 Human)

Tags

GFP

Expression

Mammalian

Mutation

bp 172 C to T; aa L58F

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pBabepuro3-p18

Plasmid

#24935

Depositor

Lynda Chin

Article

Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

Insert

p18 (CDKN2C Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only
pCMV4-SMAC-HA-eGFP

Plasmid

#67490

Purpose

Targets HA-eGFP to mitochondrial intermembrane space (IMS)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SMAC (DIABLO Human)

Tags

HA-eGFP

Expression

Mammalian

Mutation

aa1-59

Promoter

attenuated CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pFIN-EF1a-GFP:2A:CHER(HA)-WPRE

Plasmid

#44597

Depositor

Susan Semple-Rowland

Article

Verrier et al PLoS One. 2011;6(5):e20553. doi: 10.1371

Insert

EF1a-GFP:2A:CHER(HA)

Use

Lentiviral

Promoter

human elongation factor -1a (pos 2528-3971)

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pGL4.24-Cd36-enhancer8

Plasmid

#138580

Purpose

Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17923535_17924934

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 Promoter (Cd36 Mouse)

Use

Luciferase

Promoter

Minimal promoter

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pEMS2157

Plasmid

#70118

Purpose

Promoterless AAV plasmid with MCS to insert promoter for expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-MCS-EmGFP-WPRE

Use

AAV

Expression

Mammalian

Promoter

Promoterless

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARL5A-GFP

Plasmid

#67399

Purpose

Mammalian expression of hArl5 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL5A (ARL5A Human)

Tags

GFP

Expression

Mammalian

Mutation

bp 70 C to T; silent mutation

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDSHA-ARL1-Myc

Plasmid

#67353

Purpose

Mammalian expression of hArl1 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL1 (ARL1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pSico shSMYD3-C human

Plasmid

#85654

Purpose

expression of shRNA targeting human SMYD3

Depositor

Julien Sage

Article

Mazur et al Nature. 2014 Jun 12;510(7504):283-7. doi

Insert

shRNA targeting 3'UTR of SMYD3 (SMYD3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDSHA-ARF3-Myc

Plasmid

#67349

Purpose

Mammalian expression of hArf3 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF3 (ARF3 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only