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We narrowed to 8,836 results for: construct

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Showing: 8681 - 8700 of 8836 results

  2. AAV-pCBh-ABE8e-nSpCas9(D10A)[N-term] (HES1468)

    Plasmid
    #197509
    Purpose
    Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A)
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A)[N-term]-NpuN-BPNLS-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Mutation
    ABE8e mutations in TadA and nSpCas9(D10A)
    Promoter
    Cbh
    Available Since
    April 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    197509-plasmid-map-sequence-id-391242

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  5. pRSET-RCaMP1h

    Plasmid
    #42874
    Purpose
    a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby
    Depositor
    Loren Looger
    Article
    Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33
    Insert
    Red Fluorescent Calcium binding Protein
    Tags
    EK (Enterokinase) Recognition Site, His Tag (6x),…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    42874-plasmid-map-sequence-id-58020

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  9. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)

    Plasmid
    #217800
    Purpose
    Variant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promoters
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217800-plasmid-map-sequence-id-432875

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  10. G protein alpha-q-CFP

    Plasmid
    #66081
    Purpose
    This G protein alpha-q construct contains internal insertions of CFP and the EE epitope
    Depositor
    Catherine Berlot
    Article
    Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.
    Insert
    G protein alpha-q-EE-CFP (Gnaq Mouse)
    Tags
    CFP was inserted internally into the alpha-q sequ…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available Since
    July 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    66081-plasmid-map-sequence-id-118921

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  11. G protein alpha-o-YFP

    Plasmid
    #55776
    Purpose
    This G protein alpha-o construct contains internal insertions of YFP and the EE epitope
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    G protein alpha-o internally tagged with EYFP and EE epitope (Gnao1 Rat)
    Tags
    EE epitope was introduced internally with D167E a…
    Expression
    Mammalian
    Mutation
    A BglII site was removed from the 5' untrans…
    Promoter
    CMV
    Available Since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    55776-plasmid-map-sequence-id-112541

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  14. Fibronectin-human-mutated V1 O-Glycosylation site

    Plasmid
    #120406
    Purpose
    enables eukaryotic expression of human plasma fibronectin in which the third O-glycosylation site was mutated from threonine to serine
    Depositor
    Inaam Nakchbandi
    Article
    Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.
    Insert
    Fibronectin (FN1 Human)
    Expression
    Mammalian
    Mutation
    Contains complete variable region with a mutation…
    Promoter
    CMV
    Available Since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    120406-plasmid-map-sequence-id-233165

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  15. pLEX307-RAD54L2-D1315-1467-SFB

    Plasmid
    #247919
    Purpose
    Mammalian expression construct for C-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged RAD54L2 D1315-146
    Depositor
    Junjie Chen, Huimin Zhang
    Article
    Zhang et al Sci Adv. 2023 Dec 8;9(49):eadi6681. doi:
    Insert
    RAD54L2 (RAD54L2 Human)
    Use
    Lentiviral
    Tags
    S protein-Flag-Streptavidin binding peptide (SFB)
    Expression
    Mammalian
    Mutation
    deletion of amino acid 1315-1467
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    247919-plasmid-map-sequence-id-495273

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  16. pLEX307-RAD54L2-D87-291-SFB

    Plasmid
    #247914
    Purpose
    Mammalian expression construct for C-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged RAD54L2 D87-29
    Depositor
    Junjie Chen, Huimin Zhang
    Article
    Zhang et al Sci Adv. 2023 Dec 8;9(49):eadi6681. doi:
    Insert
    RAD54L2 (RAD54L2 Human)
    Use
    Lentiviral
    Tags
    S protein-Flag-Streptavidin binding peptide (SFB)
    Expression
    Mammalian
    Mutation
    deletion of amino acid 87-291
    Available Since
    Dec. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    247914-plasmid-map-sequence-id-494863

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  17. pLEX307-RAD54L2-D1-86-SFB

    Plasmid
    #247913
    Purpose
    Mammalian expression construct for C-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged RAD54L2 D1-86
    Depositor
    Junjie Chen, Huimin Zhang
    Article
    Zhang et al Sci Adv. 2023 Dec 8;9(49):eadi6681. doi:
    Insert
    RAD54L2 (RAD54L2 Human)
    Use
    Lentiviral
    Tags
    S protein-Flag-Streptavidin binding peptide (SFB)
    Expression
    Mammalian
    Mutation
    deletion of amino acid 1-86
    Available Since
    Dec. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    247913-plasmid-map-sequence-id-494860

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Showing: 8681 - 8700 of 8836 results