We narrowed to 2,107 results for: Endog
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Plasmid#129634PurposeVisualization of active RhoADepositorInsertRhoA binding doamin of PKN1(30 - 100) codon optimized
ExpressionMammalianPromoterCMVdelAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJN601
Plasmid#59790Purposezf1::yfp fusion with floxed unc-119(+) within an intron of yfp. For creating zf1::yfp knock-ins using unc-119 as a markerDepositorInsertsZF1 domain from pie-1
N-terminal half of yfp with introns
intron containing floxed unc-119 gene in reverse orientation
C-terminal half of yfp with introns
ExpressionWormAvailable SinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pLJC2-UMPS (Y37A, R155A)-3xFLAG
Plasmid#87976PurposeExpression of UMPS (Y37A, R155A) in mammalian cellsDepositorInsertUMPS (Y37A, R155A) (UMPS Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationY37A, R155APromoterCMVAvailable SinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLJC2-Rap2A-3xFLAG
Plasmid#87974Purpose3rd generation lentiviral vector for Expression of Rap2A in mammalian cellsDepositorAvailable SinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDH-AtAFB2-mTagBFP2
Plasmid#179889PurposeExpresses AtAFB2 (auxin-inducible degron system) fused to mTagBFP2 (lentiviral plasmid)DepositorInsertAtAFB2 (AFB2 Synthetic, Mustard Weed)
UseLentiviralTagsmTagBFP2ExpressionBacterial and MammalianPromoterEF1Available SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330_rActb sgRNA / hSpCas9
Plasmid#172833PurposeMammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of rActb under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV.U6gT28.13.EFS-NS.H2B-RFP
Plasmid#170388PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.DepositorInsertH2B-RFP
UseLentiviralPromoterEFS-NSAvailable SinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_Tuba1b sgRNA / hSpCas9
Plasmid#172835PurposeMammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_rSyn1 sgRNA / hSpCas9
Plasmid#172840PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-EKAR
Plasmid#181858PurposeGenetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-EKAR
TagsmRuby2 and sfGFP(1-10)ExpressionMammalianPromoterCMVAvailable SinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pChlr-1SE
Plasmid#52140PurposeEncodes module 1 with amino acids 12S-16E for recognition of nt 8GDepositorInsertPumilio homology domain amino acids 25-60 (PUM1 Human)
ExpressionBacterialMutationChanged 16Q to 16E in Module 1Available SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-2SE
Plasmid#52144PurposeEncodes module 2 with amino acids 12S-16E for recognition of nt 7GDepositorInsertPumilio homology domain amino acids 61-96 (PUM1 Human)
ExpressionBacterialMutationChanged 12N to 12S and 16Q to 16E in Module 2Available SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-3SE
Plasmid#52148PurposeEncodes module 3 with amino acids 12S-16E for recognition of nt 6GDepositorInsertPumilio homology domain amino acids 97-132 (PUM1 Human)
ExpressionBacterialMutationChanged 12C to 12S and 16Q to 16E in Module 3Available SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
Psicheck2 MERTK full length 3'UTR
Plasmid#26992DepositorInsertMERTK Full length 3' UTR (MERTK Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMammalianAvailable SinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pChlr-1SR
Plasmid#52142PurposeEncodes module 1 with amino acids 12S-16R for recognition of nt 8CDepositorInsertPumilio homology domain amino acids 25-60 (PUM1 Human)
ExpressionBacterialMutationChanged 16Q to 16R in Module 1Available SinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-1NQ
Plasmid#52141PurposeEncodes module 1 with amino acids 12N-16Q for recognition of nt 8UDepositorInsertPumilio homology domain amino acids 25-60 (PUM1 Human)
ExpressionBacterialMutationChanged 12S to 12N in Module 1Available SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-2CQ
Plasmid#52143PurposeEncodes module 2 with amino acids 12C-16Q for recognition of nt 7ADepositorInsertPumilio homology domain amino acids 61-96 (PUM1 Human)
ExpressionBacterialMutationChanged 12N to 12C in Module 2Available SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-2SR
Plasmid#52146PurposeEncodes module 2 with amino acids 12S-16R for recognition of nt 7CDepositorInsertPumilio homology domain amino acids 61-96 (PUM1 Human)
ExpressionBacterialMutationChanged 12N to 12S and 16Q to 16R in Module 2Available SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-3NQ
Plasmid#52149PurposeEncodes module 3 with amino acids 12N-16Q for recognition of nt 6UDepositorInsertPumilio homology domain amino acids 97-132 (PUM1 Human)
ExpressionBacterialMutationChanged 12C to 12N in Module 3Available SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only