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pWay21-MKP-1 FL

Plasmid

#13469

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1 FL (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pDONR223-EPHA2

Plasmid

#23926

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

EPHA2 (EPHA2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCAG:H2B-EGFP

Plasmid

#32599

Depositor

Anna-Katerina Hadjantonakis, Virginia Papaioannou

Article

Hadjantonakis et al BMC Biotechnol. 2004 Dec 24;4:33.

Insert

H2B-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-ARF

Plasmid

#19930

Depositor

Yue Xiong

Article

Zhang et al Cell. 1998 Mar 20. 92(6):725-34.

Insert

cyclin-dependent kinase inhibitor 2A p19ARF (CDKN2A Human)

Tags

myc

Expression

Mammalian

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-H-Ras_V12

Plasmid

#39504

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 (HRAS Human)

Expression

Mammalian

Mutation

G12V

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk3a2

Plasmid

#13759

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a2 (MAPK10 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
HR_G3BP1-V5-APEX2-GFP

Plasmid

#105284

Purpose

homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus

Depositor

Eugene Yeo

Article

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 soybean, Human)

Use

Human homologous recombination donor

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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InsRA-eGFP

Plasmid

#79795

Purpose

Insulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

InsRA (INSR Human)

Tags

eGFP extracellular

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-pro-IL1b

Plasmid

#73953

Purpose

Express murine pro-interleukin-1 beta in mammalian cells

Depositor

Christian Stehlik

Article

Bryan et al J Immunol. 2009 Mar 1;182(5):3173-82. do

Insert

interleukin-1 beta (Il1b Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-C1-PKCgamma-C1A

Plasmid

#21205

Depositor

Tobias Meyer

Article

Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

Insert

PKCgamma-C1A (Prkcg Rat)

Tags

GFP

Expression

Mammalian

Mutation

PKCgamma-C1A domain. (Lab plasmid WC 0018)

Available Since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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pECE-M2-PAK2 wt

Plasmid

#31663

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 (PAK2 Human)

Tags

M2

Expression

Mammalian

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.iGluSnFR3.v857.GPI

Plasmid

#178335

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV CAG iGluSnFR3 v857.GPI

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

GPI Anchor, IgK-chain, and Myc epi tag

Expression

Mammalian

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pBSSK p85 alpha (delta iSH2)

Plasmid

#13432

Depositor

Ronald Kahn

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

Insert

p85 alpha (delta iSH2) (PIK3R1 Human)

Tags

flag

Expression

Bacterial

Mutation

Dominant negative p85 alpha. The p110 alpha bindi…

Available Since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only
pET28a-HaloTag7-SnoopLigase

Plasmid

#105627

Purpose

Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with N-terminal HaloTag7

Depositor

Mark Howarth

Article

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

HaloTag7-SnoopLigase

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-CIB1-mCerulean-MP

Plasmid

#58366

Purpose

For use in light-induced protein inactivation through the interaction with CRY2

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Inserts

CIB1[1-147] (CIB1 Mustard Weed)
Camk2a[315-478] (Camk2a Rat)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pQE30-His-NDK

Plasmid

#124136

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Nucleotide diphosphate kinase (ndk Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo

Plasmid

#108101

Purpose

Lentiviral expression plasmid of cDNA with neomycin resistance gene (empty vector)

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

FLAG tag

Expression

Mammalian

Promoter

EFS promoter

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk2a1

Plasmid

#13754

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2a1 (MAPK9 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pARF1(T31N)-CFP

Plasmid

#11384

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 1 (ARF1 Human)

Tags

CFP

Expression

Mammalian

Mutation

Changed Threonine 31 to Asparagine.

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

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GSK3beta (1015)

Plasmid

#49491

Purpose

expresses human Gsk3beta

Depositor

Scott Friedman

Article

Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

Insert

GSK3beta (GSK3B Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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