We narrowed to 1,196 results for: MS2;

1. psPax2-D64V-NC-mNeon

   Plasmid

   #196509

   Purpose

   mNeon fluorescent protein fused to nucleocapsid protein in psPAX2

   Depositor

   Howard Chang

   Article

   Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

   Insert

   mNeon
   

   Use

   Tags

   mNeon

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE UbiC NLS-HA-stdMCP-stdHalo-RH1

   Plasmid

   #164614

   Purpose

   Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag and the RH1 domain of RILPL2. Used for single-molecule imaging of actin-anchored mRNA.

   Depositor

   Jeffrey Chao

   Article

   Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.

   Insert

   stdMCP-stdHalo-RH1
   

   Use

   Lentiviral

   Tags

   HA tag and NLS

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   UbiC

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-UniSAM

   Plasmid

   #99866

   Purpose

   Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0

   Depositor

   Lesley Forrester

   Article

   Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.

   Insert

   UniSAM-mCherry + U6-gRNA2.0
   

   Use

   CRISPR and Synthetic Biology; Dcas9-sam activation

   Tags

   Expression

   Mammalian

   Mutation

   BbsI sites in CDS were ablated by consensus mutat…

   Promoter

   EF1a

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tagged-IRES

   Plasmid

   #35570

   Depositor

   Marian Waterman

   Article

   Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

   Insert

   Lymphoid Enhancer Factor 1 5'UTR (LEF1 Human)
   

   Use

   Luciferase

   Tags

   Firefly Luciferase and MS2 Stem Loop

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   April 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSB700 lenti gRNA Cerulean-zhang2.0 Weiss full

   Plasmid

   #79383

   Purpose

   Cas9 gRNA variant to recruit ms2 proteins

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   Zhang 2.0 + scaffold
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG MCP-VP64

   Plasmid

   #92357

   Purpose

   CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   MCP-VP64
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCK389.AAV

   Plasmid

   #177327

   Purpose

   aTc inducible MCP-SoxS double mutant expression for CRISPRa in E.coli. Contains constitutive dCas9 and scRNA expression

   Depositor

   James Carothers

   Article

   Tickman et al Cell Syst. 2021 Nov 17. pii: S2405-4712(

   Inserts

   SoxS
   dCas9
   

   Use

   CRISPR

   Tags

   MCP (MS2 Coat Protein)

   Expression

   Mutation

   R93A,S101A

   Promoter

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. MCP-mCherry-SEC61B

   Plasmid

   #207671

   Purpose

   To express fluorescently labeled MS2 coat binding protein (MCP) fused with ER resident protein SEC61B

   Depositor

   Christine Mayr

   Article

   Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

   Insert

   SEC61B (SEC61B Human)
   

   Use

   Tags

   MCP-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQAT25

   Plasmid

   #223397

   Purpose

   T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by AtUBQ10 and sgRNA was driven by AtU3; Hygromycin for plants select.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   AtUBQ10-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYPQAT26

    Plasmid

    #223398

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by AtU3; BASTA for plants select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYPQAT27

    Plasmid

    #223399

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by 2x35s and the sgRNA was driven by AtU3; Kanamycin for plant select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYPQAT28

    Plasmid

    #223400

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and sgRNA was driven by OsU3; Hygromycin for plant select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYPQAT29

    Plasmid

    #223401

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by OsU3; BASTA for plant select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYPQAT30

    Plasmid

    #223402

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 and the sgRNA was driven by separate ZmUbi1; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-ZmUbi-gRNA scaffold 2.0-tRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MCP/PCP yellow RNA Lantern

    Plasmid

    #226096

    Purpose

    RNA tracking

    Depositor

    Jennifer Prescher

    Article

    Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

    Insert

    NLS-HA-MS2-SmBiT-IRES-PP7-Venus d12-LgBiT-FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. MCP/PCP red RNA Lantern

    Plasmid

    #226097

    Purpose

    RNA tracking

    Depositor

    Jennifer Prescher

    Article

    Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

    Insert

    NLS-HA-MS2-SmBiT-IRES-PP7-mScarlet-I-LgBiT-FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lentiSAM v2 (Puro)

    Plasmid

    #92062

    Purpose

    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Adam Karpf

    Article

    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (sgRNA) and EF1a (dCas9-VP64)

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Ef1a-MCP-huADARE488QT490A

    Plasmid

    #159922

    Purpose

    Fusion of MS2 coat protein to Human ADAR2 E488QT490A, under Ef1a promoter, with WPRE. AKA 116v5

    Depositor

    Fei Chen

    Article

    Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

    Insert

    Human ADAR2 E488QT490A (ADARB1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ141-ZmUbi-RZ-Aa3.8.4

    Plasmid

    #136375

    Purpose

    Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is used

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mADAM8 deltaMP in pQCXIP cFLAG

    Plasmid

    #19149

    Depositor

    Zena Werb

    Article

    Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

    Insert

    ADAM8 (Adam8 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    delta 228-397

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only