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Showing: 861 - 880 of 9356 results

  5. pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

    Plasmid
    #133793
    Purpose
    U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    CjeCas9 sgRNA entry vector
    Use
    Tags
    Expression
    Mammalian
    Mutation
    V2-TGAA linker sgRNA architecture from Kim et al.…
    Promoter
    U6
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133793-plasmid-map-sequence-id-268077

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  9. pAAV-CMV-FLEX-SaCas9-U6-sgKcnj6

    Plasmid
    #159913
    Purpose
    Mutagenesis of Kcnj6
    Depositor
    Larry Zweifel
    Insert
    Kcnj6 (Kcnj6 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159913-plasmid-map-sequence-id-317320

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  12. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

    Plasmid
    #223137
    Purpose
    pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223137-plasmid-map-sequence-id-447707

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  13. pX330-U6-BRD4-HaloTag-Guide-hspCas9

    Plasmid
    #228576
    Purpose
    Expression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertion
    Depositor
    Marcin Imielinski
    Insert
    BRD4 gRNA (BRD4 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228576-plasmid-map-sequence-id-457355

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  19. CMV:AsCpf1-2A-GFP-U6-tdTomato-sg

    Plasmid
    #194722
    Purpose
    Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target tdTomato
    Depositor
    Rudolf Jaenisch
    Article
    Haiting et al bioRxiv
    Insert
    AsCpf1
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    194722-plasmid-map-sequence-id-381295

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  20. CMV:AsCpf1-2A-GFP-U6-INS-sg

    Plasmid
    #194719
    Purpose
    Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hINS
    Depositor
    Rudolf Jaenisch
    Article
    Haiting et al bioRxiv
    Insert
    AsCpf1 (INS Acidaminococcus sp.)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    194719-plasmid-map-sequence-id-381224

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Showing: 861 - 880 of 9356 results