We narrowed to 9,536 results for: pho

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pDEST26 Ago2 S387A

Plasmid

#59905

Purpose

Expresses 6xHis-Ago2 (N-termi tag) S387A mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

S387A, phospho-null alanine

Promoter

CMV

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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MBP-rPKL S12TAG

Plasmid

#107155

Purpose

MBP-rPKL construct for production and purification of rat PKL pS12

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Mutation

Serine 12 to TAG for phosphoserine insertion

Promoter

PBAD

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-rPKL S113TAG

Plasmid

#107157

Purpose

MBP-rPKL S113TAG for production and purification of rat PKL pS113

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Mutation

Serine 113 changed to TAG for phosphoserine inser…

Promoter

PBAD

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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RMCE ABCDE>ala DNA-PKcs

Plasmid

#233914

Purpose

Low copy number provides expression of human DNA-PKcs with the 6 ABCDE phosphorylation sites substituted to alanine.

Depositor

Katheryn Meek

Article

Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

Insert

human DNA-PKcs ABCDE phosphorylation site mutant (PRKDC Human)

Expression

Mammalian

Mutation

phosphorylation sites 2609, 2612, 2620, 2624, 263…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-1 MKK4(EE)-MKK7a1(EE)

Plasmid

#47580

Purpose

Bacterial expression plasmid for co-expression of constitutively active MKK4 and MKK7a1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Inserts

MKK4-EE (MAP2K4 Human)
MKK7a1-EE (Map2k7 Mouse)

Expression

Bacterial

Mutation

Lacks the first 36 residues of the RefSeq protein…

Available Since

Sept. 19, 2013

Availability

Academic Institutions and Nonprofits only
pmEos2-C1 PA-PLA1 (mEos2-PA-PLA1)

Plasmid

#162878

Purpose

Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with mEos2 to perform sptPALM

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)

Tags

mEos2

Expression

Mammalian

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES_Tomato MEF2C S222D

Plasmid

#107231

Purpose

hMEF2C phosphomimetic obtained from Quikchange Lightning Mutagenesis

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C (MEF2C Human)

Use

Retroviral

Expression

Mammalian

Mutation

S222D

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-3_Flag-TET1-S-WT

Plasmid

#232942

Purpose

Expresses short isoform of TET1 in mammalian cells in its wild-type form

Depositor

Jianjun Chen

Article

Chen et al Sci Transl Med. 2023 Mar 29;15(689):eabq

Insert

TET1-S-WT (TET1 Human)

Use

Lentiviral

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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His-SUMO-Rab10 Q68L

Plasmid

#236720

Purpose

Expresses Rab10 Q68L with His tag and SUMO cleavage sequence

Depositor

Suzanne Pfeffer

Article

Claire Y. et al bioRxiv, 2025

Insert

Rab10 Q68L (RAB10 Human)

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

Q68L

Promoter

T7

Available Since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-3_Flag-TET1-S-MUT

Plasmid

#232943

Purpose

Expresses mutant form of the short isoform of TET1 in mammalian cells

Depositor

Jianjun Chen

Article

Chen et al Sci Transl Med. 2023 Mar 29;15(689):eabq

Insert

TET1-S-MUT (TET1 Human)

Use

Lentiviral

Mutation

H1652Y and D1654A

Available Since

March 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-no-Flag-TET1-S-WT

Plasmid

#232941

Purpose

Expresses short isoform of TET1 in mammalian cells in its wild-type form

Depositor

Jianjun Chen

Article

Chen et al Sci Transl Med. 2023 Mar 29;15(689):eabq

Insert

TET1-S-WT (TET1 Human)

Use

Lentiviral

Available Since

March 21, 2025

Availability

Academic Institutions and Nonprofits only

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AIMTOR A757

Plasmid

#140829

Purpose

AIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757

Depositor

Anne Bonnieu

Article

Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1

Insert

AIMTOR A757 (ULK1 Human)

Tags

NES Nuclear export signal

Expression

Mammalian

Mutation

The insert comprises only a small sequence of hum…

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRSETa mEos2

Plasmid

#20341

Purpose

bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)

Depositor

Loren Looger

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

mEos-2

Tags

His

Expression

Bacterial

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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mEos2-Lifeact-7

Plasmid

#54809

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Lifeact-mEos2

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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mEos2-MAP4-C-10

Plasmid

#57399

Purpose

Localization: Microtubules, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

MAP4

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mEos2-Integrin-Beta3-N-18

Plasmid

#57391

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Integrin-Beta3

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mEos2-Lysosomes-20

Plasmid

#57397

Purpose

Localization: Lysosome Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

LAMP1

Tags

mEos2

Expression

Mammalian

Mutation

D50E

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pTPTP alpha

Plasmid

#17691

Depositor

Xinmin Zheng

Article

Zheng et al EMBO J. 2000 Mar 1. 19(5):964-78.

Insert

PTPalpha (PTPRA Human)

Tags

HA

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
Dendra2-VASP-5

Plasmid

#57744

Purpose

Localization: Actin/Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

VASP

Tags

Dendra2

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mEos2-Cx43-7

Plasmid

#57365

Purpose

Localization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Cx43

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits