We narrowed to 16,597 results for: puromycin

1. pU6-sgGAL4-4

   Plasmid

   #46916

   Purpose

   Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter (negative control)

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Inserts

   sgGAL4-4
   Puromycin resistance and mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. SHIP2 WT pBABE-His-Xpress

   Plasmid

   #126584

   Purpose

   Retroviral infection of MEF cells (puromycin selection)

   Depositor

   Christophe Erneux

   Article

   Zhang et al Cell Signal. 2007 Oct;19(10):2194-200. d

   Insert

   SHIP2 (INPPL1 Human)
   

   Use

   Retroviral

   Tags

   His Xpress (N-terminal)

   Available Since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXPR_BRD003 sgKEAP1-2

   Plasmid

   #74986

   Purpose

   sgKEAP1-2, puromycin selection

   Depositor

   William Hahn

   Article

   Krall et al Elife. 2017 Feb 1;6:e18970. doi: 10.7554

   Insert

   KEAP1
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiGuide-Puro-NCOA7g1 (BB22)

   Plasmid

   #139457

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a / U6

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiGuide-Puro-NCOA7g2 (BB23)

   Plasmid

   #139458

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a / U6

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBMN-puro-P2A-FRB-Fis1

   Plasmid

   #135297

   Purpose

   stably express FBR-Fis1 in mammalian cells with puromycin selection

   Depositor

   Richard Youle

   Article

   Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

   Insert

   FRB-Fis1 (FIS1 Human)
   

   Use

   Retroviral

   Tags

   FRB

   Expression

   Mammalian

   Mutation

   Fis1 tail (aa 93-152)

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDF1-ADAMTS18 Wt

   Plasmid

   #29552

   Depositor

   Yardena Samuels

   Article

   Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

   Insert

   ADAMTS18 (ADAMTS18 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Wild type

   Available Since

   July 22, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pSicoR-Ef1a-mCh-Puro-GFPi

   Plasmid

   #31848

   Depositor

   Bruce Conklin

   Article

   Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

   Insert

   GFP RNAi
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Promoter

   U6 for shRNA; Ef1alpha for mCherry-puromycin

   Available Since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-tetpA-hNEUROG2-iRPT

   Plasmid

   #140764

   Purpose

   Expresses iRFP713, PuroR and rtTAM2 in mammalian cells, with TRE-hNEUROG2

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Neurosci Res. 2019 Oct 3. pii: S0168-010

   Inserts

   iRFP713
   PuroR
   rtTAM2
   NEUROG2 (NEUROG2 Human)
   

   Expression

   Mammalian

   Available Since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICE-HA-CtIP-siR-N289A-H290A

    Plasmid

    #82032

    Purpose

    Plasmid for constitutive or doxy-inducible expression of N289A+H290A mutant of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    CtIP (RBBP8 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Endonuclease-dead mutant: N289A+H290A CtIP; Mutat…

    Promoter

    CMV-tet

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-CMV-Flag-PolB(K244A/T304I)-Puro

    Plasmid

    #177144

    Purpose

    Lentiviral vector expressing Flag-PolB(K244A/T304I) and a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pICE-HA-CtIP-siR-S664A-S679A-S745A

    Plasmid

    #82031

    Purpose

    Plasmid for constitutive or doxy-inducible expression of S664A+S679A+S745A human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    CtIP (RBBP8 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Non phosphorylatable mutations on 3 of the main A…

    Promoter

    CMV-tet

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSicoR-Ef1a-mCh-Puro-Luci

    Plasmid

    #31850

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Insert

    Luciferase RNAi
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available Since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. double_sgRNA targeting e3 and e7

    Plasmid

    #190689

    Purpose

    sgRNAs targeting enhancer 3 and 7 of MYC separately

    Depositor

    Stanley Qi

    Article

    Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

    Insert

    sgRNAs targeting enhancer 3 and 7 of MYC separately
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    BFP and Puromycin

    Expression

    Mammalian

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXPR_BRD003 sgCIC-2

    Plasmid

    #74967

    Purpose

    sgCIC-2, puromycin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    CIC (CIC Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-CMV-cGAS R71/75E C396/7A-HA

    Plasmid

    #130920

    Purpose

    Expresses human cGAS with R71/75E and C396/397A point mutations; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    human cGAS R71/75E C396/7A
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa 71 and 75 mutated to glutamate; aa 396 and 397…

    Promoter

    CMV

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSUPER-retro-puro-tetO-Mek1-shRNA

    Plasmid

    #53179

    Purpose

    Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (Map2k1 Mouse)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pTBL649 CHYRON2 integration construct

    Plasmid

    #126446

    Purpose

    To integrate the CHYRON2 locus at AAVS1 in human cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6-CHYRON2 hgRNA
    pCMV-puro
    

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV-RUNX1-4A

    Plasmid

    #238534

    Purpose

    Used for overexpression of human RUNX1 in mammalian cells. Contains mutations that prevent phosphorylation at some sites of the translated protein.

    Depositor

    Diane Krause

    Article

    Kwon et al Blood. 2024 Oct 24;144(17):1800-1812. do

    Inserts

    RUNX1 (RUNX1 Human)
    Puromycin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    S249A, S266A, T273A, S276A

    Promoter

    MSCV and PGK

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSCV-RUNX1-4D

    Plasmid

    #238535

    Purpose

    Used for overexpression of human RUNX1 in mammalian cells. Contains RUNX1 phosphomimetic mutations.

    Depositor

    Diane Krause

    Article

    Kwon et al Blood. 2024 Oct 24;144(17):1800-1812. do

    Inserts

    RUNX1 (RUNX1 Human)
    Puromycin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    S249D, S266D, T273D, S276D

    Promoter

    MSCV and PGK

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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