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pPfetFn-Target_v2

Plasmid

#199564

Purpose

gRNA expression for FnCas12a in E. coli and clostridia

Depositor

Philippe Lambin

Article

Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

Insert

SacB

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Pthl_fU

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBx-Spas-sgRNA-Kan

Plasmid

#105233

Purpose

Plasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Kanamycin marker for P. putida and P. fluorescens

Depositor

Kristala Prather

Article

Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

Insert

S. pasteurianus sgRNA

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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qTAG-C-ultraID-Puro

Plasmid

#207726

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-moxGFP-Zeo

Plasmid

#207732

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pWS3799-gRNA-Csy4-template

Plasmid

#182827

Purpose

SpCas9 gRNA-Csy4 template

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

SpCas9 gRNA scaffold + Csy4 sequence

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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qTAG-KO-Blast

Plasmid

#227263

Purpose

Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a 2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB302

Plasmid

#107694

Purpose

OsActin Promoter-LwaCas13a-HSP Terminator

Depositor

Daniel Voytas

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

Cas13a

Expression

Plant

Promoter

OsActin

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pX330S-7

Plasmid

#58783

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQ134B2.0

Plasmid

#167158

Purpose

Golden Gate entry vector to express the 4th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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TwinStrep-SUMO-NLS-BhCas12b_v4

Plasmid

#158615

Purpose

For protein expression of BhCas12b_v4

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

NLS-BhCas12b_v4

Tags

6xHIS-TwinStrep-SUMO

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pU3-gRNA

Plasmid

#53063

Purpose

Transcription of guide RNA (gRNA) in rice cells

Depositor

Caixia Gao

Article

Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

Insert

OsU3 promoter + gRNA scaffold

Use

CRISPR; Plant expression

Promoter

rice U3

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQ142-ZmUbi-tRNA

Plasmid

#158578

Purpose

Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 and pYPQ132-tRNA2.0; assembly of 2 gRNAs

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Maize ubiquitin 1

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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EC2_1_dCas9_sgRNA

Plasmid

#163710

Purpose

Yeast low copy plasmid with dCas9 and sgRNA expression cassette

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

dCas9

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLsCas13a-gRNA-NT

Plasmid

#164857

Purpose

Constitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-NT

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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PL6-CS-WR

Plasmid

#122512

Purpose

construction for sgRNA transcription with Plasmodium falciparum U6 promotor

Depositor

Lubin Jiang

Article

Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

Type

Empty backbone

Use

CRISPR

Promoter

PfU6

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pM370

Plasmid

#173178

Purpose

(Empty Backbone) gRNA-12xMBS

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

gRNA-12xMBS

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pJAT77

Plasmid

#204295

Purpose

pJAT plasmid with multiple cloning site for introducing custom payload. No U6 promoter. Restriction sites present to introduce flanking U6 promoters.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-C-miRFP670nano3-Zeo

Plasmid

#207735

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Zeo-miRFP670nano3

Plasmid

#207702

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-miRFP670nano3 cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pX330A_dCas9-1x7

Plasmid

#63601

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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