We narrowed to 16,083 results for: nes

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DCT.VV.Orf78

Plasmid

#12766

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf78

Expression

Mammalian

Available Since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf90

Plasmid

#12778

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf90

Expression

Mammalian

Available Since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
pCLXSN-ING2

Plasmid

#13293

Depositor

Curtis Harris

Article

Pedeux et al Mol Cell Biol. 2005 Aug . 25(15):6639-48

Insert

ING2 (INGX Human)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pRET.IIS.IRES-EGFP

Plasmid

#1838

Depositor

Philip Leder

Article

Ishida et al Nucleic Acids Res 1999 Dec 15;27(24):e35

Type

Empty backbone

Use

Cre/Lox and Retroviral

Expression

Mammalian

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
SAR1BH79G-BFP

Plasmid

#250873

Purpose

Transient mammalian expression of SAR1BH79G-BFP

Depositor

Thomas Melia

Article

Fuller et al eLife 2025 14:RP107316

Insert

SAR1B (SAR1B Human)

Tags

TagBFP

Expression

Mammalian

Mutation

H79G

Available Since

March 17, 2026

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA0650

Plasmid

#240487

Purpose

CRISPRi-mediated Knockdown of RPA0650

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-BSD-FLAG-La-Cas9

Plasmid

#245102

Purpose

Inserts tetracycline-inducible codon optimized, nuclceus targeted FLAG-Cas9 into T. brucei cells at the rDNA spacer

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

3xFLAG-La-Cas9

Use

CRISPR

Tags

3xFLAG-La

Mutation

codon optimized for T. brucei

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST14-Cys-SpyCatcher (Min)

Plasmid

#216713

Purpose

Expresses truncated minimal form of SpyCatcher with free cysteine for labeling in bacteria. See also Li...Howarth, J Mol. Biol., 2014

Depositor

Alex Dunn

Article

Zhong et al Cell Rep Methods. 2022 Sep 2;2(9):100278

Insert

Cys-SpyCatcher (Min)

Tags

6xHis

Expression

Bacterial

Mutation

See Li...Howarth, J. Mol. Biol., 2014

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pRD439

Plasmid

#168465

Purpose

For the insertion pf NLS-mTurquoise2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS-mTurquoise2-H-NSdbd-NLS

Expression

Mammalian

Promoter

CMV promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9noSsrA

Plasmid

#102285

Purpose

Modified from pCas9-CR4 (Addgene: 62655) to remove the ssrA tag from Cas9.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Mutation

removed ssrA tag

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-H2-Q1-alpha3-domain

Plasmid

#15863

Depositor

Peter Mombaerts

Article

Ishii et al J Neurosci. 2008 Mar 5. 28(10):2332-41.

Insert

H2-Q1 (H2-Q1 Mouse)

Available Since

May 6, 2008

Availability

Academic Institutions and Nonprofits only
pSA071 Cas9-BFP

Plasmid

#249132

Purpose

Overexpression of SpCas9-BFP and blasticidin resistance gene.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

Cas9-TagBFP

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pPB9v2

Plasmid

#210029

Purpose

Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas9
rtta-T2A-puro

Use

CRISPR

Tags

SV40 NLS and nucleoplasmin NLS

Expression

Mammalian

Promoter

EF-1α and TRE3G

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-SpCas9D10A nickase

Plasmid

#216736

Purpose

Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)

Depositor

Vincent Dion

Article

Alvaro et al bioRxiv

Insert

Cas9-D10A nickase

Use

AAV and CRISPR

Expression

Mammalian

Mutation

D10A

Promoter

EF-1-alpha core

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ULK1-1-835-mcherry

Plasmid

#249729

Purpose

Expressing human ULK1-1-835 with a MBP tag in N-terminal and a mcherry tag in C-terminal

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ULK1 (ULK1 Human)

Tags

MBP and Mcherry

Expression

Mammalian

Mutation

1-835

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pAAVLINK-EFS-HA-5'-KIF1A

Plasmid

#244342

Purpose

5' AAVLINK plasmid for KIF1A expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

KIF1A (KIF1A Human)

Use

AAV

Tags

HA

Promoter

EFS

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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AiP11712 - pAAV-hsA2-AiE0219h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN1712)

Plasmid

#230101

Purpose

AiE0219h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minRho

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15263 - pAAV-AiE0219h-minBG-iCre(R297T)-BGHpA (Alias: CN5263)

Plasmid

#230406

Purpose

AiE0219h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Enterovirus D68 (EV-D68) strain EV-D68 STL 2014 12 3C protease

Plasmid

#228644

Purpose

Bacterial expression of the D68 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

Enterovirus D68 3C protease

Tags

His-SUMO

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pME Actin Vhh Halo Tag (JDW 1223)

Plasmid

#224498

Purpose

Gateway compatible middle entry clone containing an Actin nanonbody fused to a flexible linker and Halo Tag (For visualizing actin, actin chromobody)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-Halo-Tag

Use

Gateway Cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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