We narrowed to 11,436 results for: 110

1. px330-MRPsg2

   Plasmid

   #87190

   Purpose

   disruption of human RMRP gene via CRISPR-Cas9, guide 2

   Depositor

   Thomas Cech

   Article

   Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

   Insert

   human MRP RNA CRISPR guide #2 (RMRP Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBstSK+IRES-V5-IPpoI

   Plasmid

   #206321

   Purpose

   A plasmid that facilitates production of mRNA encoding V5 epitope tagged I-PpoI

   Depositor

   Brian McStay

   Article

   van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

   Insert

   IRES element-V5 epitope tag- IPpoI open reading frame
   

   Use

   Mrna production

   Tags

   V5-IPpoI fusion protein

   Expression

   Mutation

   Promoter

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEN-R2-mCsfGFP-L3

   Plasmid

   #178602

   Purpose

   It can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.

   Depositor

   Frederica Theodoulou

   Article

   Zhang et al Plant Physiol. 2019 Jun;180(2):718-731.

   Insert

   Tandem fluorescent protein timers (tFTs)
   

   Use

   Entry vector for gateway cloning

   Tags

   mCherry superfolder GFP

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSBL_DK193

   Plasmid

   #156424

   Purpose

   Insect cell expression of 3a(Q57H)-sfGFP, needs to be introduced into baculoviral bacmid

   Depositor

   Stephen Brohawn

   Article

   Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

   Insert

   SARS-CoV-2 3a protein (Q57H)
   

   Use

   Tags

   sfGFP-His

   Expression

   Insect

   Mutation

   Q57H

   Promoter

   Polyhedrin

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. mVenus-CENPB-N-22

   Plasmid

   #56325

   Purpose

   Localization: Nucleus/Centromeres, Excitation: 515, Emission: 528

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   CENPB (CENPB Human)
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   aa 1-169 of CENPB (DNA Binding Domain)

   Promoter

   CMV

   Available since

   Dec. 19, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pGL3-RbE2Fmut

   Plasmid

   #32391

   Depositor

   Julien Sage

   Article

   Burkhart et al Mol Cell Biol. 2010 Apr;30(7):1729-45. E

   Insert

   Rb promoter (Rb1 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutated E2F binding site: TTTCCCGC --> TTTACATC

   Promoter

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pCMV-Nxph4-6A-HA

   Plasmid

   #128103

   Purpose

   Express HA tagged Nxph4-6A

   Depositor

   Huda Zoghbi

   Article

   Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

   Insert

   Nxph4 (Nxph4 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM33_PTK2_Focal-AT

   Plasmid

   #178485

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   PTK2_Focal-AT (PTK2 Human, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETM33_ZYMND11_coiled-coil MYND

   Plasmid

   #178493

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   ZYMND11_coiled-coil MYND (ZMYND11 Human, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTaCas9_6

    Plasmid

    #91169

    Purpose

    Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLPC-MYC.Mm.RAP1.delta.MYB

    Plasmid

    #184660

    Purpose

    Retroviral expression of mouse RAP1 delta MYB

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    rap1
    

    Use

    Retroviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    rap1 delta MYB

    Promoter

    CMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLPC-MYC.Mm.RAP1.delta.COIL

    Plasmid

    #184659

    Purpose

    Retroviral expression of mouse RAP1 delta COIL

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    pLPC
    

    Use

    Retroviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    rap1 delta COIL

    Promoter

    CMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLPC-MYC.Mm.RAP1.delta.BRCT

    Plasmid

    #184658

    Purpose

    Retroviral expression of mouse Rap1 delta BRCT

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    RAP1
    

    Use

    Retroviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    rap1 delta BRCT

    Promoter

    CMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLPC-MYC.Mm.RAP1.delta.RCT

    Plasmid

    #184661

    Purpose

    Retroviral expression of mouse RAP1 delta RCT

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    rap1
    

    Use

    Retroviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    rap1 delta RCT

    Promoter

    CMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. GAL 103Q+ProCFPp303

    Plasmid

    #15575

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt103Q + Pro (HTT Human)
    

    Use

    Tags

    CFP

    Expression

    Yeast

    Mutation

    Proline-rich region present

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

16. phNFL-E396K

    Plasmid

    #132595

    Purpose

    Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E E396K mutation

    Depositor

    Anthony Brown

    Article

    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

    Insert

    NEFL (NEFL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1186G>A; Glu396Lys

    Promoter

    CMV

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEN_TT Tgfbr3-HA

    Plasmid

    #58497

    Purpose

    Gateway entry vector for an inducible HA-tagged mouse Tgfbr3

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    Tgfbr3_HA (Tgfbr3 Mouse)
    

    Use

    Entry vector

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pET21a(+) LdtMt2 (131-408)

    Plasmid

    #59337

    Purpose

    Expresses LdtMt2 for L,D-transpeptidation

    Depositor

    SeWon Suh

    Article

    Kim et al Acta Crystallogr D Biol Crystallogr. 201

    Insert

    LdtMt2 (131-408)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pMOD_A1810

    Plasmid

    #91040

    Purpose

    Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_dead (D10A + H840A), Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-TaCas9_dead (D10A + H840A)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, H840A

    Promoter

    ZmUbi

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

20. PXNGW

    Plasmid

    #48258

    Purpose

    SPLIT-GFP assay in plants

    Depositor

    Wolf Frommer

    Article

    Kim et al Plant Cell. 2009 Apr;21(4):1305-23. doi:

    Type

    Empty backbone

    Use

    Binary gateway vector

    Tags

    5xHIS and YFP aa1-172

    Expression

    Mutation

    Promoter

    35S

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only