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pLPC FLAG hRap1 delta BR delta CT

Plasmid

#13257

Depositor

Titia de Lange

Article

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 delta Br delta CT (TERF2IP Human)

Use

Retroviral

Tags

FLAG

Mutation

contains hRap1 aa2-40 + 96-270 +331-399 and the p…

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pBS-KS-attB1-2-PT-SA-SD-2-2xTY1-V5

Plasmid

#61257

Purpose

CRISPR-RMCE, second step attB plasmid, intron phase 2, exchange dsRed with 2xTY1 and V5 tags cassette

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

2xTY1-V5

Use

CRISPR

Tags

TY1 and V5

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

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mV-VfAU1-LOV_226

Plasmid

#58686

Purpose

Expresses mVenus-fusion protein of VfAU1-LOV domain (Vaucheria frigida Aureochrome 1)

Depositor

Harald Janovjak

Article

Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

Insert

mV-VfAU1-LOV

Expression

Mammalian

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pBAD*RFPEC2

Plasmid

#17823

Depositor

Jay Keasling

Article

Pfleger et al Biotechnol Bioeng. 2005 Dec 5. 92(5):553

Insert

E. coli optimized red flourescent protein (RFPec2)

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

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RAD Module G8-C1

Plasmid

#38208

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

SET Integrase
Bxb1 Excisonase
RESET Integrase

Use

Synthetic Biology; Biobrick cloning vector

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pFtnA-Ypet

Plasmid

#98280

Purpose

mammalian expression of FtnA-Ypet

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

FtnA

Tags

Ypet

Expression

Mammalian

Promoter

CMV

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pSEVA43-GG

Plasmid

#208014

Purpose

for cloning homology arms, with GFP as counter selection marker

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Insert

msfGFP

Promoter

14G

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pG1AK-PheB

Plasmid

#71738

Purpose

Modular shuttle vector for Geobacillus and E. coli

Depositor

Tom Ellis

Article

Reeve et al ACS Synth Biol. 2016 Jul 1.

Insert

PheB

Use

Synthetic Biology; Shuttle vector

Expression

Bacterial

Promoter

pRplsWT

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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Gal-Rad51

Plasmid

#33230

Depositor

Hannah Klein

Article

Shah et al Mol Cell. 2010 Sep 24;39(6):862-72.

Insert

Rad51 (RAD51 Budding Yeast)

Expression

Yeast

Promoter

Gal1

Available Since

Feb. 7, 2012

Availability

Academic Institutions and Nonprofits only
pRK DPC4 deltaC Flag

Plasmid

#12628

Depositor

Rik Derynck

Article

Zhang et al Nature. 1996 Sep 12. 383(6596):168-72.

Insert

DPC4 delta C (SMAD4 Human)

Tags

flag

Expression

Mammalian

Mutation

C-terminal truncated DPC4 (aa 1-511)

Available Since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pAL-pgi_NT

Plasmid

#74067

Purpose

pAL374 plasmid carrying the pgi (NT) sgRNA, targeting the non-template strand of pgi, SpecR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

sgRNA pgi (NT)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

ptac

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pBW213ara-hrpS

Plasmid

#61435

Purpose

pBAD18-cm encoding rbs33-hrpS-ter

Depositor

Martin Buck, Baojun Wang

Article

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

rbs33-hrpS-ter

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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BSP606

Plasmid

#122251

Purpose

expression vector for mECitrine under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mECitrine

Expression

Worm

Promoter

EFT3

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAG499-FRB-N-frFAST

Plasmid

#160059

Purpose

Expresses FRB-N-frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

FRB-N-frFAST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Trex2

Plasmid

#79246

Purpose

Expression vector for Trex2, which is a non-processive 3' exonuclease.

Depositor

Jeremy Stark

Article

Bennardo et al PLoS Genet. 2009 Oct;5(10):e1000683. doi

Insert

Trex2 (Trex2 Mouse)

Expression

Mammalian

Promoter

pCAGGS

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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MYR-CPH1S-o_445

Plasmid

#82684

Purpose

Expresses CPH1S-o domain (Synechocystis Phytochrome 1) preceded by a MYR signal and a restriction site

Depositor

Harald Janovjak

Article

Reichhart et al Angew Chem Int Ed Engl. 2016 May 17;55(2

Insert

MYR-CPH1S-o

Expression

Mammalian

Available Since

Nov. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pGT-Ah2

Plasmid

#122569

Purpose

MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

beta-lactamase/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Pink

Plasmid

#160445

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Pink chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Pink

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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p70rg 15

Plasmid

#17831

Depositor

Jay Keasling

Article

Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

Insert

E. coli optimized red flourescent protein (RFPec), GFPuv

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pBW115lac-hrpR

Plasmid

#61434

Purpose

pAPT110 encoding rbsH-hrpR-ter

Depositor

Martin Buck, Baojun Wang

Article

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

rbsH-hrpR-ter

Expression

Bacterial

Promoter

pLac

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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