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C453-E24: attB4-UbCp>-attB5

Plasmid

#162980

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the human Ubiquitin C promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human Ubiquitin C promoter

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E23: attB5r-mRuby3-nostop-attB1r

Plasmid

#162945

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mRuby3 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Ruby3 fluorescent protein

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E22: attB5r-mClover3-nostop-attB1r

Plasmid

#162944

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mClover3 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Clover3 fluorescent protein

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C238-E02: attB2r-BGHpolyA-attB3

Plasmid

#162916

Purpose

Gateway attB2r/attB3 entry clone containing a polyA sequence for transcription termination

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Bovine growth hormone polyadenylation sequence

Use

Synthetic Biology

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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AL12R-EF-Tu

Plasmid

#161013

Purpose

PP7-tagged reporter driven by the Arabidopsis EF-Tu promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Expression

Plant

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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Cry2PHR - IBAR

Plasmid

#176602

Purpose

Cry2PHR with C-terminal IBAR domain

Depositor

Robert Hughes

Article

Wurz et al J Biol Chem. 2022 Aug 18;298(10):102388.

Insert

Cry2PHR-mCherry-IBAR

Expression

Mammalian

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDD398

Plasmid

#91832

Purpose

AID NT-tag linker donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Auxin-inducible degron (AID)

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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ARF17 pBluescript

Plasmid

#12068

Depositor

David Bartel

Article

Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

Insert

ARF17 (ARF17 Mustard Weed)

Expression

Bacterial

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
C453-E23: attB4-TRE3Gp>-attB5

Plasmid

#162979

Purpose

Gateway attB4/attB5 entry clone for inducible mammalian expression using the TRE3G promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Tet-regulated TRE3G minimal promoter

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pUG-UBC2

Plasmid

#104667

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC2 (UBC2 Mustard Weed)

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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mRuby2-NT-N1

Plasmid

#54612

Purpose

Localization: N1 Cloning Vector, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Type

Empty backbone

Tags

mRuby2-NT

Expression

Mammalian

Available Since

June 22, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pMDC32-SEIPIN3

Plasmid

#96975

Purpose

Express Arabidopsis SEIPIN3 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN3 (AT2G34380 Mustard Weed)

Expression

Plant

Promoter

35S

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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C512-E04: attB5r-mCherry-nostop-attB1r

Plasmid

#162941

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mCherry fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Cherry fluorescent protein

Use

Synthetic Biology

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pXN-FBLWLF-GAL80

Plasmid

#26265

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

GAL80 (GAL80 Budding Yeast)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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AtFLS2pro::GUS

Plasmid

#87167

Purpose

expression pattern of AtFLS2

Depositor

Silke Robatzek

Article

Beck et al J Exp Bot. 2014 Dec;65(22):6487-98. doi:

Insert

AT5G46330 promoter

Tags

GUS

Promoter

AtFLS2

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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C413-E31: attB4-TRE3Gp>-attB1r

Plasmid

#162924

Purpose

Gateway attB4/attB1r entry clone for inducible mammalian expression using the TRE3G promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Tet-regulated TRE3G minimal promoter

Use

Synthetic Biology

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pFETCh_ZNF503

Plasmid

#135735

Purpose

Donor vector for 3' FLAG tag of human ZNF503

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF503 Homology arms (ZNF503 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBLIC-neo

Plasmid

#45198

Purpose

a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells. Neo selection.

Depositor

Priyamvada Rai

Article

Patel et al BMC Biotechnol. 2012 Jan 16;12:3. doi: 1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only
pUG-UBC27

Plasmid

#104692

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC27 (UBC27 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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NHA-Ago4

Plasmid

#21537

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago4 (AGO4 Human)

Tags

NHA

Expression

Mammalian

Available Since

Aug. 6, 2009

Availability

Academic Institutions and Nonprofits only