We narrowed to 8,957 results for: plat

1. pAAV-SEPT_Cdk2-T160A-siR

   Plasmid

   #73975

   Purpose

   HDR template to generate Cdk2-T160A-siR

   Depositor

   Steven Dowdy, Manuel Kaulich

   Article

   Kaulich et al Nucleic Acids Res. 2015 Apr 20;43(7):e45

   Inserts

   Cdk2-LHA-siR (CDK2 Human)
   Cdk2-RHA-T160A (CDK2 Human)
   

   Use

   AAV

   Mutation

   T160 mutated to Ala and siRNA target site mutated

   Available Since

   March 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF3296

   Plasmid

   #144772

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   HIF1A (HIF1A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF0390

   Plasmid

   #143875

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SMARCA4 (SMARCA4 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TFORF1964

   Plasmid

   #142518

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CUX1 (CUX1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIG-836_NY-ESO-1_TCR_FAS/OX40

   Plasmid

   #207499

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   FAS/OX40, NY-ESO-1_TCR (FAS Human)
   

   Use

   CRISPR

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIG-833_NY-ESO-1_TCR_FAS/CD28

   Plasmid

   #207496

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   FAS/CD28, NY-ESO-1_TCR (FAS Human)
   

   Use

   CRISPR

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAav-TP53-T2A-BirA*

   Plasmid

   #115652

   Purpose

   rAAV-based donor template for genome engineering of the TP53 protein C-terminus containing a T2A-BirA* module and a selection cassette

   Depositor

   Sven Eyckerman

   Article

   Vandemoortele et al J Proteome Res. 2018 Dec 10. doi: 10.102

   Inserts

   TP53 (TP53 Human)
   TP53 (TP53 Human)
   T2A
   BirA*
   

   Use

   AAV

   Tags

   BirA* and T2A

   Mutation

   Homology region 1 and Homology region 2

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF1960

   Plasmid

   #142517

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CUX1 (CUX1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TFORF1962

   Plasmid

   #142900

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CUX1 (CUX1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TFORF1963

    Plasmid

    #142299

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CUX1 (CUX1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TFORF0386

    Plasmid

    #142611

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SMARCA4 (SMARCA4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR223-PDGFRL

    Plasmid

    #23591

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PDGFRL (PDGFRL Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pDONR P2R-P3-IRES_ECFP

    Plasmid

    #48354

    Purpose

    Contributes an IRES-ECFP cassette as the 3’-module during MultiSite Gateway cloning of a bi-cistronic mRNA shared with a two-part fusion protein encoded by the 5′- and middle modules.

    Depositor

    Anna Planas, Tomas Santalucia

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    IRES_ECFP
    

    Use

    Gateway entry vector

    Mutation

    ECFP translation is mediated by an IRES. Contains…

    Promoter

    none

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-SEPT_Cdk2-T160E-siR

    Plasmid

    #73976

    Purpose

    HDR template to generate Cdk2-T160E-siR

    Depositor

    Steven Dowdy, Manuel Kaulich

    Article

    Kaulich et al Nucleic Acids Res. 2015 Apr 20;43(7):e45

    Inserts

    Cdk2-LHA-siR (CDK2 Human)
    Cdk2-RHA-T160E (CDK2 Human)
    

    Use

    AAV

    Mutation

    T160 mutated to Glu and siRNA target site mutated

    Available Since

    March 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV KI Cloning Vector_hSyn mTagBFP2-CAAX2

    Plasmid

    #236245

    Purpose

    Cloning template for knock-in based strategy with U6 promoter, gRNA and donor tag cloning cassettes, and a fluorescent marker for the plasma membrane under hSyn promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mTagBFP2 fused to the plasma membrane targeting sequence CAAX2
    

    Use

    AAV

    Tags

    mTagBFP2

    Promoter

    U6 and human Synapsin1

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pML390_YWHAQ-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227925

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    YWHAQ homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pML389_YWHAB-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227926

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    YWHAB homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pML600_COG8-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227911

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    COG8 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pML357_CAV1-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227914

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    CAV1 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pML361_DCP1A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227918

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    DCP1A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge