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pMT Lifeact-TagBFP

Plasmid

#126664

Purpose

Inducible expression of Lifeact-TagBFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Lifeact-TagBFP fusion

Expression

Insect

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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AAV Cag Flex H2B Gcamp6s reverse

Plasmid

#74157

Purpose

Calcium Sensor

Depositor

Loren Looger

Insert

Gcamp6s

Use

AAV

Tags

H2B

Promoter

SpeI

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pRpoH-Wasabi

Plasmid

#86107

Purpose

mWasabi reporter gene driven by RpoH promoter

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Insert

mWasabi

Tags

6xHis

Expression

Bacterial

Promoter

pRpoH

Available Since

Feb. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLacIq-Wasabi

Plasmid

#86112

Purpose

mWasabi reporter gene driven by LacIq promoter

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Insert

mWasabi

Tags

6xHis

Expression

Bacterial

Promoter

pLacIq

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMAL-c2x-MT2

Plasmid

#32102

Depositor

David DeRosier, Chris Mercogliano, Cristina Risco Ortiz

Article

Mercogliano et al J Struct Biol. 2007 Oct;160(1):70-82. Ep

Insert

MT (2x) (Mt1 Mouse)

Tags

MBP

Expression

Bacterial

Promoter

P-lac

Available Since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only
pEBB-TB

Plasmid

#36099

Depositor

Ezra Burstein

Article

Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

Type

Empty backbone

Tags

BT-tag

Expression

Mammalian

Promoter

EF1a

Available Since

June 15, 2012

Availability

Academic Institutions and Nonprofits only

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pL

Plasmid

#64177

Purpose

harbours marker gene LEU2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pQE-WT-T3-R5-RRVH

Plasmid

#63623

Purpose

T3-R5-RRVH RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

T3 RRVH RNAP

Mutation

T745K, N748D, L749M, M750I, G753R, H772R, E775V, …

Promoter

T5/Lac

Available Since

July 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pGC219

Plasmid

#19687

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Phlh-12

Use

Flp/frt

Expression

Worm

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pPSSUB-101

Plasmid

#26580

Depositor

Ron Weiss

Article

Basu et al Proc Natl Acad Sci U S A. 2004 Apr 27. 1

Insert

destabilized GFP

Expression

Bacterial

Mutation

S2R, C-terminal extension RPAANDENYA(LVA)

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pNL-EGFP/TRETightdU3

Plasmid

#18660

Depositor

Jakob Reiser

Article

Pluta et al J Gene Med. 2005 Jun . 7(6):803-17.

Insert

EGFP

Use

Lentiviral

Available Since

July 8, 2008

Availability

Academic Institutions and Nonprofits only

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pBS cTbx3 (CT#23)

Plasmid

#13920

Depositor

Cliff Tabin

Article

Logan et al Development. 1998 Aug . 125(15):2825-35.

Insert

Tbx3 (TBX3 Chicken)

Expression

Bacterial

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
CMVTO-TVMVP

Plasmid

#179659

Purpose

Tobacco Vein Mottling Viral Protease

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

TVMVP

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pQE-WT-KIF-R5-IRH

Plasmid

#63629

Purpose

K1F-R5-IRH RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

KIF-R5-IRH RNAP

Mutation

L749I, Q754S, R756N, I761V, H772R, and Q786H

Promoter

T5/Lac

Available Since

June 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMT p85-GFP

Plasmid

#126660

Purpose

Inducible expression of p85-GFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

PI3K SH2 containing subunit

Expression

Insect

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET21b-RL018A

Plasmid

#42137

Depositor

Sheref Mansy

Article

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

mCerulean-mCherry

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 4, 2013

Availability

Academic Institutions and Nonprofits only

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pDnaK-Wasabi

Plasmid

#86110

Purpose

mWasabi reporter gene driven by DnaK promoter

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Insert

mWasabi

Tags

6xHis

Expression

Bacterial

Promoter

pDnaK

Available Since

Feb. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pME-NLS-tdMCP

Plasmid

#86245

Purpose

Middle entry vector for inserting promotors for tissue specific expression of NLS-tdMCP

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Type

Empty backbone

Use

Unspecified

Tags

NLStdMCP

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pET His10-Src42a

Plasmid

#126674

Purpose

Expression of His10-Src42a for purification from E. coli

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Src42a

Expression

Bacterial

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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PKC eta dNPS

Plasmid

#21245

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC eta (Prkch Mouse)

Tags

HA

Expression

Mammalian

Mutation

delta 1-165

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only