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pFVTd5

Plasmid

#167520

Purpose

Broad-Host range expression vector, RSF1010 origin, VanRAM repressor, PVanCC promoter, tdKatushka2, Gentamicin Resistance Marker

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanRAM-tdKatushka2

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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TC646

Plasmid

#164892

Purpose

Pdpy30::loxP::mcherry::let-858terminator::loxP::GFP

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

GFP

Expression

Worm

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFCK(0.4)GW

Plasmid

#27229

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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LP580

Plasmid

#90205

Purpose

pCMV-myc with cDNA encoding HsTRAPPC13 full length

Depositor

Lotte Pedersen

Article

Verdier et al Methods Mol Biol. 2016;1454:15-33. doi:

Insert

TRAPPC13 (TRAPPC13 Human)

Tags

Myc

Expression

Mammalian

Mutation

full length protein, no mutations

Promoter

CMV

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pKCyR5

Plasmid

#149463

Purpose

Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CymR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET23b-proMMP-7

Plasmid

#29524

Depositor

William Parks

Article

Ra et al J Biol Chem. 2009 Oct 9. 284(41):27924-3

Insert

proMMP-7 (MMP7 Human)

Expression

Bacterial

Available Since

April 29, 2011

Availability

Academic Institutions and Nonprofits only
pFA6a-natMX-tetO-Pcyc1-3xFLAG

Plasmid

#41021

Depositor

Michael Nick Boddy

Article

Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

Insert

tetO7-Pcyc1-3xFLAG

Use

N-terminal tagging/promoter replacement

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP6

Plasmid

#80662

Purpose

Encodes the synthetic coiled coil peptide SYNZIP6

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP6

Tags

6xHis

Expression

Bacterial

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP5

Plasmid

#80661

Purpose

Encodes the synthetic coiled coil peptide SYNZIP5

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP5

Tags

6xHis

Expression

Bacterial

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBLxR5

Plasmid

#167504

Purpose

Broad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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CMVTO-Gbp6-TM-Che

Plasmid

#180022

Purpose

Membrane-bound anti-GFP nanobody conjugated to mCherry

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Gpb6 nanobody

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pFLtR5

Plasmid

#167505

Purpose

Broad-Host range expression vector, RSF1010 origin, TetR repressor, PLtetO-1 promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

TetR-RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLtetO-1

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pHH04_32_sRNA-A_Sp_coD

Plasmid

#120900

Purpose

the component plasmid of a regulated device

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

Inserts

pECF32 promoter [i.e., Pecf32_1122 (-60 to -1)]
sRNA-A

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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TSC2-3A (S1337A/S1341A/S1345A)

Plasmid

#29360

Depositor

Kunliang Guan

Article

Inoki et al Cell. 2003 Nov 26. 115(5):577-90.

Insert

TSC2 (Tsc2 Rat)

Expression

Mammalian

Mutation

S1337A/S1341A/S1345A (equivalent to S1381/S1385/S…

Available Since

Jan. 20, 2012

Availability

Academic Institutions and Nonprofits only
pAW12

Plasmid

#110224

Purpose

integration of ura4+-loxM3 cassette

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Insert

ura4+ (ura4 Fission Yeast)

Use

Cre/Lox

Mutation

See depositor comments below.

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFLlR5

Plasmid

#149481

Purpose

Broad-Host range expression vector, RSF1010 origin, LacI repressor, PLlacO-1 promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LacI-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLlacO-1

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFVR5

Plasmid

#149489

Purpose

Broad-Host range expression vector, RSF1010 origin, VanR(AM) repressor, PVanCC promoter, mRFP, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFCyR5

Plasmid

#149480

Purpose

Broad-Host range expression vector, RSF1010 origin, CymR(AM) repressor, PCymRC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CymR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pB-Neo-rTOM20-GFP

Plasmid

#210331

Purpose

Overexpression of rTOM20

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

rTOM20 (Tomm20 Rat)

Tags

GFP

Expression

Mammalian

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL294

Plasmid

#167834

Purpose

Encodes OptoLAC1 circuit

Depositor

Jose Avalos

Article

Lalwani et al Nat Chem Biol. 2021 Jan;17(1):71-79. doi

Insert

lacI

Use

Synthetic Biology

Tags

ssrA (LAA)

Expression

Bacterial

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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