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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22,696 results for: cas9

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Showing: 8901 - 8920 of 22696 results

  1. MSP2262

    Plasmid
    #70703
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A & H557A in SaCas9
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    70703-plasmid-map-sequence-id-127765

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  5. pGEM-3XP3.RFP-tan

    Plasmid
    #83474
    Purpose
    RFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.
    Depositor
    Patricia Wittkopp
    Publication
    Lamb et al Fly (Austin). 2016 Aug 5:1-12.
    Insert
    3XP3.RFP-tan
    Use
    CRISPR
    Expression
    Bacterial and Insect
    Promoter
    3XP3 eye-expression Promoter
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    83474-plasmid-map-sequence-id-159168

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  11. pSABEd

    Plasmid
    #133968
    Purpose
    Expresses ABEd CRISPR base editor and gRNA scaffold in Streptomyces
    Depositor
    Yuhui Sun
    Publication
    Zhong et al bioRxiv 630137
    Insert
    ABEd
    Use
    CRISPR and Synthetic Biology
    Available since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    133968-plasmid-map-sequence-id-266624

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  16. pJAT11

    Plasmid
    #204290
    Purpose
    For inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.
    Depositor
    David Stern
    Publication
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-DsRed
    Use
    CRISPR
    Promoter
    ie1
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204290-plasmid-map-sequence-id-405507

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  20. pCfB6682

    Plasmid
    #106152
    Purpose
    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6627, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Publication
    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/
    Type
    Empty backbone
    Expression
    Yeast
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    106152-plasmid-map-sequence-id-205326

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Showing: 8901 - 8920 of 22696 results