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pBTK1062

Plasmid

#236186

Purpose

BsaI compatible kanamycin resistance cassette

Depositor

Jeffrey Barrick

Publication

VanDieren et al MicroPubl Biol. 2025 Aug 2;2025:10.17912

Insert

Kanamycin resistance cassette

Expression

Bacterial

Promoter

CP25

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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Vph1-2GFP-URA

Plasmid

#207009

Purpose

Integration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

VPH1

Tags

2xGFP

Expression

Yeast

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-4493NES(Booster-PKA)

Plasmid

#184710

Purpose

PKA biosensor

Depositor

Michiyuki Matsuda

Publication

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

Booster-PKA

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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3H-α-synuclein

Plasmid

#205714

Purpose

Mammalian expression of 3H-alpha-synuclein

Depositor

Randy Schekman

Publication

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

alpha-synuclein (SNCA Human)

Tags

thermostable three helix-bundle protein 3H

Expression

Mammalian

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEN_TT BirA*

Plasmid

#192309

Purpose

Gateway entry vector for an inducible 3xFLAG-BirA*

Depositor

Kevin Janes

Publication

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BirA*

Use

Gateway Cloning

Tags

3xFLAG

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot6-D491A_AF

Plasmid

#148659

Purpose

Bacterial Expression of HsNot6-D491A

Depositor

Elisa Izaurralde

Publication

Chen et al Nucleic Acids Res. 2021 Jun 21;49(11):64

Insert

HsNot6-D491A (CNOT6 Human)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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IRE1-2EGFP-ter-kanMX4

Plasmid

#184766

Purpose

Integration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4.

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

IRE1 (IRE1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 Intergenic control guide 2

Plasmid

#193585

Purpose

sgRNA control; induces CAS9 cutting in an intergenic region

Depositor

William Hahn

Publication

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgRNA control

Use

Lentiviral

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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LLP752_L2pV1_AND_B3RT_FRT_Rluc_2IBF_lacZ

Plasmid

#192396

Purpose

To act as a 2-input state of the AND gate.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP726_L2_pV01_0I_TMV-tGFP_TCTP-fLUC

Plasmid

#192406

Purpose

To be a control plasmid that has no Rluc repression (replaced by turboGFP) and should reflect true off state of the circuit.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::Turbo GFP

Use

Synthetic Biology

Tags

N7

Expression

Plant

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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THOC6

Plasmid

#156085

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

THOC6 (THOC6 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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RALYL-1

Plasmid

#155877

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

RALYL (RALYL Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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CIR1

Plasmid

#155452

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CIR1 (CIR1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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ND6-L1397-N_UGI-NES_left

Plasmid

#187845

Purpose

expresses the left-side half ND6-UGI-NES in mammalian cells

Depositor

Chengqi Yi

Publication

Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

Insert

SOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–hivNES–SOD2 3'UTR

Expression

Mammalian

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-CaCYP722C(pYL803)

Plasmid

#178291

Purpose

Expresses CaCYP722C from C. annuum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-CaCYP722C-TCYC1

Depositor

Yanran Li

Publication

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

CaCYP722C

Expression

Yeast

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-LjCYP722C(pYL795)

Plasmid

#178292

Purpose

Expresses LjCYP722C from L. japonicus in Saccharomyces cerevisiae. Centromeric TRP, PGPD-LjCYP722C-TCYC1

Depositor

Yanran Li

Publication

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

LjCYP722C

Expression

Yeast

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-VuCYP722C(pYL1070)

Plasmid

#178293

Purpose

Expresses VuCYP722C from V. unguiculata in Saccharomyces cerevisiae. Centromeric TRP, PGPD-VuCYP722C-TCYC1

Depositor

Yanran Li

Publication

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

VuCYP722C

Expression

Yeast

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-SlCYP722C(pYL1071)

Plasmid

#178294

Purpose

Expresses SlCYP722C from S. lycopersicum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-SlCYP722C-TCYC1

Depositor

Yanran Li

Publication

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

SlCYP722C

Expression

Yeast

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-TpCYP722C(pYL851)

Plasmid

#178295

Purpose

Expresses TpCYP722C from T. pratense in Saccharomyces cerevisiae. Centromeric TRP, PGPD-TpCYP722C-TCYC1

Depositor

Yanran Li

Publication

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

TpCYP722C

Expression

Yeast

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-CsCYP722C(pYL877)

Plasmid

#178296

Purpose

Expresses CsCYP722C from C. sinensis in Saccharomyces cerevisiae. Centromeric TRP, PGPD-CsCYP722C-TCYC1

Depositor

Yanran Li

Publication

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

CsCYP722C

Expression

Yeast

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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