We narrowed to 1,383 results for: piggyBac

1. MF64_PB-TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA

   Plasmid

   #176624

   Purpose

   TF B self-activation cassette in MultiFate-2.1 and MultiFate-2.3

   Depositor

   Michael Elowitz

   Article

   Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

   Insert

   TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
   

   Use

   Synthetic Biology

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. MF85_PB-14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA

   Plasmid

   #176629

   Purpose

   TF C self-activation cassette in MultiFate-3

   Depositor

   Michael Elowitz

   Article

   Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

   Insert

   14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA
   

   Use

   Synthetic Biology

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJAM1.17_PB-STEAP3-PGK-Blast

   Plasmid

   #205562

   Purpose

   Express human STEAP3

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   STEAP3 (STEAP3 Human)
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CAGGS-MCS-NLS-3XFLAG-V5

   Plasmid

   #196383

   Purpose

   Empty backbone vector to insert cDNA for strong expression in mammalian cells

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Type

   Empty backbone

   Tags

   3xFLAG and V5

   Expression

   Bacterial and Mammalian

   Promoter

   CAGGs

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-TetON-dual-SunTag-Hygro

   Plasmid

   #121119

   Purpose

   Endogenous gene activation with the SunTag CRISPR activation system

   Depositor

   Pablo Navarro

   Article

   Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

   Insert

   dCas9-GCN4x10_scFv-VP64
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Tet-ON

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-PNGT3-d2EGFP_CMV-mCherry

   Plasmid

   #213765

   Purpose

   Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

   Depositor

   Gaetano Gargiulo

   Article

   Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

   Insert

   PNGT#3-d2EGFP
   

   Use

   Synthetic Biology

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. vYM082_PB-pEF1s(min)-3xNLS-Citrine-T2A-iaaH(Hygro)

   Plasmid

   #160044

   Purpose

   For expressing iaaH to convert auxin precursors (IAM or NAM) to auxin (IAA or NAA)

   Depositor

   Michael Elowitz

   Article

   Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

   Insert

   iaaH (iaaH )
   

   Use

   Synthetic Biology

   Tags

   3xNLS-Citrine

   Expression

   Mammalian

   Promoter

   pEF1s

   Available Since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. BII-TOP-tdT

   Plasmid

   #133395

   Purpose

   Piggybac vector for canonical Wnt signaling reporter

   Depositor

   Blair Madison

   Article

   Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

   Insert

   tdTomato-NLS
   

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   SuperTOPFlash

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFH2.101_PB-EPN11-MCP-T2A-eGFP-PGK-Blast

   Plasmid

   #205546

   Purpose

   Express EPN11-MCP-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN11-MCP
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MF82_PB-TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

    Plasmid

    #176626

    Purpose

    TF A self-activation cassette in MultiFate-2.2 and MultiFate-3

    Depositor

    Michael Elowitz

    Article

    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

    Insert

    TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
    

    Use

    Synthetic Biology

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PB-CT3G-CERP2-MG-IRES2-mCherry

    Plasmid

    #175507

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mCherry (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G-mCMV

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPBbsr2-Lifeact-miRFP703

    Plasmid

    #178530

    Purpose

    Expresses Lifeact-miRFP703 in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Lifeact-miRFP703
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJAM1.18_PB-SDC4-PGK-Blast

    Plasmid

    #205563

    Purpose

    Express human SDC4

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    SDC4 (SDC4 Human)
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. OA-1067B

    Plasmid

    #200252

    Purpose

    pBac-U6-gRNA(doublesex)-3xp3-tdTomato

    Depositor

    Omar Akbari

    Article

    Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

    Insert

    4 gRNAs targeting Doublesex
    

    Expression

    Insect

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFH2.104_PB-EPN24-T2A-eGFP-PGK-Blast

    Plasmid

    #205549

    Purpose

    Express EPN24-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. BII-ChPtW-EGFP

    Plasmid

    #133400

    Purpose

    Piggybac vector for constitutive expression of EGFP

    Depositor

    Blair Madison

    Article

    Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

    Insert

    EGFP
    

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-Antares2

    Plasmid

    #120868

    Purpose

    Piggybac transposon plasmid for live animal optical imaging

    Depositor

    Jordan Green

    Article

    Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

    Insert

    pcDNA3-Antares2
    

    Expression

    Mammalian

    Mutation

    Antares2 amplicon

    Promoter

    CAG Promoter

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-CT3G-ERP2-MG-IRES2-mNeonGreen

    Plasmid

    #175506

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G-mCMV

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MF81_PB-TRE3G-12x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

    Plasmid

    #176625

    Purpose

    TF A self-activation cassette in MultiFate-2.1

    Depositor

    Michael Elowitz

    Article

    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

    Insert

    TRE3G-12x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
    

    Use

    Synthetic Biology

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB-EEA-g1-PGK-Puro

    Plasmid

    #102908

    Purpose

    PiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    EEA-guide1-PGK-Puro
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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