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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,240 results for: spl

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Showing: 881 - 900 of 4240 results

  4. AAVS1-iSAM

    Plasmid
    #211495
    Purpose
    AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa
    Depositor
    Antonella Fidanza, Lesley Forrester
    Publication
    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    UniSAM insert
    TET-ON
    Neo Resistance
    Insulator
    Use
    CRISPR and Synthetic Biology
    Tags
    T2A-mCherry Tag
    Expression
    Mammalian
    Promoter
    EF1a, NA, Splicing acceptor, and TREGV promoter
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    211495-plasmid-map-sequence-id-454077

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  6. CAG sHRPb-FKBP-post mGRASP

    Plasmid
    #73146
    Purpose
    Small fragment of split HRP and FKBP fused to the extracellular terminus of the "post mGRASP" scaffold published by Magee, in which the majority of the NLG1 extracellular domain is deleted.
    Depositor
    Alice Ting
    Publication
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sHRPb-FKBP-post mGRASP
    Tags
    HA tag
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73146-plasmid-map-sequence-id-134519

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  7. FKBP-CD28TMD-NTEVp(H75E)

    Plasmid
    #137829
    Purpose
    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75E mutant TEV protease
    Depositor
    Joshua Leonard
    Publication
    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP-CD28TMD-NTEVp(H75E)
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    Mutated Histidine 75 to Glutamic Acid in NTEVp fr…
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    137829-plasmid-map-sequence-id-311953

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  9. DDX41-GFP

    Plasmid
    #175494
    Purpose
    Total RNA was isolated from TF-1 cells and converted to cDNA using SuperScript III (Invitrogen). The full DDX41 coding sequence was cloned into pcDNA3.1 (Invitrogen) with an EGFP c-terminal insert.
    Depositor
    Evan Braunstein
    Publication
    Li et al Haematologica. 2016 Jun;101(6):e228-31.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DDX41 (DDX41 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175494-plasmid-map-sequence-id-345045

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  11. DT12A

    Plasmid
    #80416
    Purpose
    Expresses 12 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats
    Depositor
    Thomas Cooper
    Publication
    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human DMPK exons 11-15 with 12 CTG repeats (DMPK Human)
    Expression
    Mammalian
    Available since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80416-plasmid-map-sequence-id-457373

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  12. pUBC_HA_2xPP7-VP64

    Plasmid
    #68418
    Purpose
    Transient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    PP7(deltaFG)
    Use
    Synthetic Biology
    Tags
    HA and VP64
    Expression
    Mammalian
    Mutation
    PP7: [delta]67_74 (non-aggregating)
    Promoter
    human UbC
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68418-plasmid-map-sequence-id-125550

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  13. pAAVS1-Puro-CAG-mNG2(1-10)

    Plasmid
    #206042
    Purpose
    Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7
    Insert
    AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
    Use
    CRISPR and Synthetic Biology; Donor template
    Expression
    Mammalian
    Available since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    206042-plasmid-map-sequence-id-411117

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  15. pCB6-X:31HA

    Plasmid
    #74371
    Purpose
    Mammalian expression of wild-type HA from the X:31 strain of influenza virus
    Depositor
    Judith White
    Publication
    Qiao et al Mol Biol Cell. 1999 Aug;10(8):2759-69.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HA
    Expression
    Mammalian
    Available since
    April 13, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. pJYDNp

    Plasmid
    #162451
    Purpose
    Positive control plasmid carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid serves as an expression control.
    Depositor
    Gideon Schreiber
    Publication
    Zahradník et al bioRxiv Preprint 2020
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Aga2p-eUnaG2
    Use
    Shuttle vector bacteria/yeast
    Tags
    HA and myc
    Promoter
    GAL1
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162451-plasmid-map-sequence-id-321446

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  20. pUC19-TSV (linearized in VP2)

    Plasmid
    #70032
    Purpose
    pUC19-TSV (linearized in VP2) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the VP2-coding region
    Depositor
    Mariet Feltkamp
    Publication
    van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024.
    Insert
    TSV full genome, linearized in VP2 gene
    Use
    Cloning vector
    Expression
    Bacterial
    Available since
    Dec. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    70032-plasmid-map-sequence-id-129213

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Showing: 881 - 900 of 4240 results