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pEGFP-H1.1

Plasmid

#32894

Depositor

Michael Hendzel

Article

Th'ng et al J Biol Chem. 2005 Jul 29;280(30):27809-1

Insert

Histone H1.1 (H1-1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNAHygro(+)LAMP2AS

Plasmid

#86146

Purpose

Anti sense for LAMP2

Depositor

Janice Blum

Article

Zhou et al Immunity. 2005 May;22(5):571-81.

Insert

LAMP2a (LAMP2 Human)

Expression

Mammalian

Mutation

contains first 525 bases of LAMP cDNA

Promoter

CMV

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-VSVG

Plasmid

#80606

Purpose

Mammalian expression vector that expresses a VSV-G protein with a FLAG tag near the N-terminus of the protein

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

VSV-G

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pET-Galectin-8 FL

Plasmid

#68805

Purpose

Express Galectin-8 FL protein in Bacterial cells

Depositor

Hyun Kyu Song

Article

Kim et al Nat Commun. 2013;4:1613. doi: 10.1038/nc

Insert

Galectin-8 (LGALS8 Human)

Tags

N-terminal 6-histidine-tag

Expression

Bacterial

Mutation

No mutation

Promoter

T7

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
pCAG-GBP6-10gly-VPminx4

Plasmid

#50796

Purpose

Expression of a GFP-dependent transcription factor component in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GBP6-VPminx4 activation domain fusion protein

Expression

Mammalian

Promoter

CAG

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Nr5a1

Plasmid

#41081

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Nr5a1 (Nr5a1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO + minimal CMV

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pDuet TDP-43 Q331K

Plasmid

#27465

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Coexpression

Expression

Bacterial

Mutation

Glutamine 331 mutated to Lysine (Q331K)

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Rheb1 shRNA #2

Plasmid

#26626

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Rheb1 shRNA (RHEB Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Nov. 17, 2010

Availability

Academic Institutions and Nonprofits only
pME18S-3HA-mULK1

Plasmid

#22896

Depositor

Masaaki Muramatsu

Article

Yan et al Biochem Biophys Res Commun. 1998 May 8.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Tags

3xHA

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-miR17

Plasmid

#21113

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

miR-17 (MIR17 Human)

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only
CMV-H3.3-YFP

Plasmid

#8693

Depositor

Kami Ahmad

Article

Janicki et al Cell 2004 Mar 5;116(5):683-98.

Insert

H3.3A (His3.3A Fly)

Tags

YFP

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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p043 mTcf-4 B

Plasmid

#11031

Depositor

Ramesh Shivdasani

Article

Lee et al J Biol Chem. 1999 Jan 15. 274(3):1566-72

Insert

Tcf-4 (Tcf7l2 Mouse)

Use

Xenopus, avian, and zebrafish expression

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pGL3Luc-5XNF-kappaB

Plasmid

#185695

Purpose

Reporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').

Depositor

Esther López-Bayghen

Article

Hernandez-Melchor et al ASN Neuro. 2022 Jan-Dec;14:1759091422111

Insert

NF-kappaB (NFKB1 Human)

Use

Luciferase

Promoter

NF-kappaB binding site

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3/td5StayGold(c4)=GianCreg

Plasmid

#212022

Purpose

For labeling the Golgi apparatus. c4 is the appendage for N-terminal tagging with td5StayGold. ‘GianCreg’ is an abbreviation for giantin C-terminal region. ‘=’ denotes a linker [(GGGGS)3].

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GianCreg (GOLGB1 Human)

Tags

td5StayGold(c4)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-ATF5-PROX1-HNF1A

Plasmid

#149725

Purpose

expressing three liver promoting transcription factors (ATF5, PROX1, HNF1A)

Depositor

Baisong Lu

Article

Lu et al Biomaterials. 2021 Feb;269:120668. doi:

Insert

ATF5-PROX1-HNF1A (ATF5 Human)

Use

Lentiviral

Tags

P2A, T2A

Promoter

CMV

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_WT-PolyA

Plasmid

#112284

Purpose

Fluorescently labels WT human YAP1-2gamma isoform (504 aa)

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

WT human YAP1-2gamma

Promoter

EF-1 alpha

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-mTrim21ΔRING-Box

Plasmid

#105517

Purpose

Expression of mCherry-tagged mouse Trim21 with a deleted RING-box in mammalian cells

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

deletion of RING domain (aa124-462)

Promoter

CMV

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCLIPf-H2B Control Plasmid

Plasmid

#101134

Purpose

Expression of CLIP-tagged Histone H2B in mammalian cells

Depositor

New England Biolabs, Ana Egana

Insert

Histone H2B (H2BC11 Human)

Tags

CLIP-tag (CLIPf)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pCCLc-MNDU3-Luciferase-PGK-EGFP-WPRE

Plasmid

#89608

Purpose

Expression of Luciferase in human cells. 3rd generation lentiviral vector

Depositor

Fernando Fierro

Article

Beegle et al Stem Cells. 2015 Jun;33(6):1818-28. doi:

Insert

Luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

MNDU3

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Nup35

Plasmid

#87342

Purpose

To express human Nup35 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

nucleoporin 35 (NUP35 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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