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pSG 3/4

Plasmid

#63868

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UGA near N-term

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pSG 853

Plasmid

#63873

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UAA near 5' end

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pSG 163

Plasmid

#63871

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UAG near 5' end

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pENTR1a-jAspSnFR3-mRuby3

Plasmid

#203491

Purpose

jAspSnFR3-mRuby3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3

Use

Gateway entry vector

Tags

His-tag and mRuby3

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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p5xEGFP-N1

Plasmid

#122165

Purpose

Expression of 5xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

5xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAW-SpyCas1

Plasmid

#113248

Purpose

E. coli expression vector for His-MBP-S. pyogenes Cas1

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Cas1

Tags

6xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAW-SpyCas2

Plasmid

#113249

Purpose

E. coli expression vector for His-MBP-S. pyogenes Cas2

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Cas2

Tags

6xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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p7xEGFP-N1

Plasmid

#122167

Purpose

Expression of 7xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

7xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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p5204

Plasmid

#168181

Purpose

bacteria reporter

Depositor

Michael Hensel

Article

Schulte et al Commun Biol. 2021 May 4;4(1):520. doi: 1

Insert

PEM7::dsred PcypD::sfgfp (frameshift)

Expression

Bacterial

Mutation

frameshift sfGFP

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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pBT239

Plasmid

#27494

Depositor

Liqun Luo

Article

Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

Insert

TRE-tdTomato-myc

Use

Mouse Targeting

Tags

Myc

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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p9xEGFP-N1

Plasmid

#122169

Purpose

Expression of 9xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

9xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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p11xEGFP-N1

Plasmid

#122171

Purpose

Expression of 11xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

11xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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Ube2d2

Plasmid

#31410

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2D2 Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pICH44199

Plasmid

#50286

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Potato Virus X)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pWZ414-F13

Plasmid

#23056

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pLPC TRF2 deltaB

Plasmid

#18006

Depositor

Titia de Lange

Article

Karlseder et al Science. 2002 Mar 29. 295(5564):2446-9.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Mutation

Deleted Basic Domain (aa1-44)

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pEF/myc/ER FLIPglu-3.2mDelta13V

Plasmid

#18019

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16A S112A Delta13

Tags

ECFP and Venus

Expression

Mammalian

Mutation

F16A, S112A in binding protein

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pGH314_wtGFP S65T, Q80H

Plasmid

#85407

Purpose

lentiviral expression vector for wtGFP S65T-Q80H

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

wtGFP S65T, Q80H

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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porcupine - Xenopus

Plasmid

#39772

Depositor

Norbert Perrimon

Article

Kadowaki et al Genes Dev. 1996 Dec 15;10(24):3116-28.

Insert

porcupine (porcn Frog)

Expression

Bacterial

Available Since

Jan. 3, 2013

Availability

Academic Institutions and Nonprofits only
pSG lac10

Plasmid

#63875

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

-1 frameshift near 5' end

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only