We narrowed to 27,806 results for: gfp

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pCherryGFP-CoV2-Inframe-stop

Plasmid

#177620

Purpose

Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. GFP is in-frame with mCherry. Thus GFP translation is prevented by stop codon within FSE (negative control).

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pBGSA C5aR1-EGFP

Plasmid

#62612

Purpose

Constitutive expression of C5aR1-EGFP in mammalian cells

Depositor

Heini Miettinen

Article

Suvorova et al Traffic. 2005 Feb;6(2):100-15.

Insert

C5aR1 (C5AR1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

SRalpha

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ9663 - ACTB GFP-P2A CLIP Donor

Plasmid

#202688

Purpose

CLIP donor template for integrating GFP into ACTB locus. Encodes GFP with ACTB homology arms.

Depositor

Stanley Qi

Article

Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

Insert

CopGFP

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pNKX2-5-T2A-GFP-PGK-Puro

Plasmid

#203607

Purpose

Donor plasmid to create 3' knockin of NKX2-5 with T2A-GFP

Depositor

Matthew Birket

Article

Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

Insert

NKX2-5-T2A-GFP-PGK-Puro-NKX2-5 (NKX2-5 Human)

Use

Human targeting

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE-BI GFP10-RHOB/RBD-GFP11

Plasmid

#182231

Purpose

Inducible coexpression of GFP10-RHOB and RBD-GFP11 domain

Depositor

Stéphanie Cabantous

Article

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Inserts

ras homolog family member B (RHOB Human)
rhotekin RHO binding domain (RBD)

Use

Lentiviral

Tags

GFP10 (split-GFP tripartite) and GFP11 (M4)

Promoter

TRE bidirectional

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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CAG-BFP-sesRNA-tTA2-WPRE-pA

Plasmid

#192070

Purpose

Expression of BFP, sesRNA in mammalian cells, with tTA2 as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for tdTom

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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nCas9-PPE

Plasmid

#140445

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

Insert

nCas9(H840A)-M-MLV

Use

CRISPR

Expression

Plant

Mutation

H840A for Cas9; D200N, T306K, W313F, T330P and L…

Promoter

maize Ubiquitin-1

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-sgRNA-lib 2.0

Plasmid

#89638

Purpose

sgRNA expression in mammalian cells after Gateway cloning

Depositor

Wensheng Wei

Article

Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-mPKR-FLAG-IG

Plasmid

#101792

Purpose

Retroviral vector encoding C-terminally FLAG tagged mouse PKR

Depositor

Seiichi Oyadomari

Article

Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

Insert

Pkr (Eif2ak2 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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Wei lab paired-guide RNA (pgRNA) CRISPR–Cas9 library for human long non-coding RNAs (lncRNAs)

Pooled Library

#89640

Purpose

Designed for genomic deletion screening of functional lncRNAs (long non-coding RNAs) using lentiviral pgRNAs (paired-guide RNAs).

Depositor

Wensheng Wei

Article

Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

Expression

Mammalian

Use

Lentiviral

Available Since

May 17, 2017

Availability

Academic Institutions and Nonprofits only
peCALWY-NB

Plasmid

#22239

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY non-binding variant

Expression

Mammalian

Mutation

C284S, C287S, C413S and C416S mutations linker c…

Available Since

Jan. 11, 2010

Availability

Academic Institutions and Nonprofits only

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pDest472 SHP-1 WT

Plasmid

#21804

Depositor

Stephen Shaw

Article

Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

Insert

SHP-1 (PTPN6 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only
pMX-HA-UbvG08-IRES-GFP

Plasmid

#75030

Purpose

Retroviral vector with ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53)

Depositor

Daniel Durocher

Article

Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

Insert

Ubiquitin

Use

Retroviral

Tags

HA and IRES-eGFP

Mutation

UbvG08 with I44A mutation and no terminal Glycines

Available Since

June 30, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-ERK2 T183AY185F

Plasmid

#118299

Purpose

Expression of Rat ERK2

Depositor

Philip Stork

Article

Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

Insert

ERK2 T183AY185F (Mapk1 Rat)

Tags

GFP

Expression

Mammalian

Mutation

T183AY185F

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pQE-60NA-proinsulin-mEGFP

Plasmid

#160464

Purpose

Encodes a fusion of human proinsulin and the monomeric enhanced EGFP. This construct can be used as a negative control for superfolder activity in bacteria

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

proinsulin-mEGFP

Expression

Bacterial

Promoter

T5

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pExp-sfGFP

Plasmid

#112573

Purpose

Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag

Depositor

Marko Hyvönen

Type

Empty backbone

Tags

8xHis, Strep II, and sfGFP

Expression

Bacterial

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-2A-eGFP(W58X) pcDNA3.1(-)

Plasmid

#172229

Purpose

Expresses an mCherry-2A-eGFP dual reporter that contains a UAG codon in position 58 (W58X) of eGFP, in mammalian cells

Depositor

Joshua Rosenthal

Article

Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

Insert

mCherry-2A-eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLP-mOlig2-ires-gfp

Plasmid

#49399

Purpose

mammalian bicistronic expression of mouse Olig2 and EGFP

Depositor

Randall McKinnon

Insert

Olig2 (Olig2 Mouse)

Use

Cre/Lox; Acceptor vector

Tags

EGFP and IRES

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR221-H1-sgGFP1-U6-sgGFP2-7SK-sgGFP3

Plasmid

#87906

Purpose

sgRNA targeting GFP

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

sgGFP1, sgGFP2, sgGFP3

Expression

Mammalian

Promoter

H1, U6, 7SK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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FUW-hR3-EGFP

Plasmid

#119231

Purpose

Plasmid encodes full-length human VEGFR-3 gene fused in-frame with EGFP cloned in lentiviral vector.

Depositor

Kari Alitalo

Insert

VEGFR3-EGFP (FLT4 Human)

Use

Lentiviral

Tags

EGFP

Promoter

P hUbC

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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