Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 14,057 results for: Mpl

Clear keywords
Showing: 9021 - 9040 of 14057 results

  1. pATP416-­pluxO5-­ptetO7-­crtYBI

    Plasmid
    #165975
    Purpose
    Expresses carotenoid biosynthesis gene in response to homoserine lactone and doxycycline in yeast expressing LuxTA and rtTA
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:
    Inserts
    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    Use
    Synthetic Biology
    Expression
    Yeast
    Mutation
    C1281A, G1698A and C66A, C1359T
    Promoter
    Synthetic promoter (pluxO5), Synthetic promoter (…
    Available Since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    165975-plasmid-map-sequence-id-328841

    Enlarge

  4. pPICZ:mTREK-1_cryst

    Plasmid
    #133269
    Purpose
    P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322) fused to a C-terminal GFP
    Depositor
    Dan Minor
    Article
    Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d
    Insert
    Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
    Tags
    SNS- 3C_cleavage_site-TAAA-GFP
    Expression
    Yeast
    Mutation
    aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    133269-plasmid-map-sequence-id-291942

    Enlarge

  6. pcDNA5-FRT-TO-Nsp7-Nsp8-HF_GC3opt

    Plasmid
    #157693
    Purpose
    mammalian expression of C-terminally His8-Flag tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp7-Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157693-plasmid-map-sequence-id-307835

    Enlarge

  7. pMP108 Nanog-Venus-2a-mCherry

    Plasmid
    #159743
    Purpose
    Nanog targeting vector to insert fluorescent reporter of protein and gene expression levels from endogenous locus in mouse embryonic stem cells. Please see depositor comments for more detail.
    Depositor
    Sergi Regot
    Article
    Pokrass et al Dev Cell. 2020 Nov 9;55(3):328-340.e5. d
    Insert
    Nanog homeobox (Nanog Mouse)
    Use
    Mouse Targeting
    Tags
    Venus-2a-mCherry
    Available Since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    159743-plasmid-map-sequence-id-330621

    Enlarge

  9. FB013

    Plasmid
    #119710
    Purpose
    TU of the HSVtk negative marker for F2dU domesticated into pUPD2 with GGAG/TACT barcodes (reverse orientation). Used for gene KO with dual selec. According to FungalBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Negative selection marker HSVtk (Pgpda:HSVtk:Ttub)
    Use
    Synthetic Biology; Domestication of dna parts for…
    Available Since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119710-plasmid-map-sequence-id-237145

    Enlarge

  11. Epac-S-H96

    Plasmid
    #170342
    Purpose
    CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.
    Insert
    CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    170342-plasmid-map-sequence-id-344641

    Enlarge

  12. IGFALS-bio-His

    Plasmid
    #52180
    Purpose
    Expresses full-length Insulin-like growth factor-binding protein complex ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    IGFALS (IGFALS Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52180-plasmid-map-sequence-id-80544

    Enlarge

  18. pGAPTrap-Luc2-IRESMeo

    Plasmid
    #82509
    Purpose
    Vector targeting the Firefly Luciferase (Luc2) gene to the GAPDH locus of human cells. It is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Meo gene.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    Luc2-IRESMeo
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    82509-plasmid-map-sequence-id-156578

    Enlarge

  19. pSF3-Flag-Cbir-Cdc25C S216A_Myc-Nbir-14-3-3e

    Plasmid
    #90011
    Purpose
    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C S216A and N-terminally tagged NBir[2-256]-14-3-3e
    Depositor
    Julien Béthune
    Article
    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.
    Inserts
    CBirA*-Cdc25C S216A
    NBirA*-14-3-3e
    Use
    Retroviral; Flp/frt
    Tags
    FLAG and Myc
    Expression
    Mammalian
    Mutation
    Ser 216 to Ala
    Promoter
    CMV
    Available Since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    90011-plasmid-map-sequence-id-201243

    Enlarge

  20. pmCherry-3xNLS-OptoJNKi-dsm

    Plasmid
    #89743
    Purpose
    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11
    Depositor
    Michael Courtney
    Article
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    NLS-OptoJNKi dark-state mutant
    Use
    Fluorescent protein
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    C450A mutation of AsLov2Jalpha
    Promoter
    CMV
    Available Since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89743-plasmid-map-sequence-id-192503

    Enlarge

Showing: 9021 - 9040 of 14057 results