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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,648 results for: grn

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Showing: 9021 - 9040 of 15648 results

  3. pResQ shFEN3 3XF-FEN1 DeltaC

    Plasmid
    #17754
    Depositor
    Sheila Stewart
    Insert
    Flap Endonuclease I (FEN1 Human)
    Use
    Lentiviral and RNAi
    Tags
    Triple Flag
    Expression
    Mammalian
    Mutation
    Deletion of C-terminal 20 amino acids from FEN1
    Available since
    April 29, 2008
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  4. pNA0304

    Plasmid
    #165612
    Purpose
    Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)
    Depositor
    Marcus Noyes
    Publication
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    NotI site replaced the CAN1 spacer in parent vect…
    Promoter
    SNR52
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165612-plasmid-map-sequence-id-331070

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  7. pTPH413

    Plasmid
    #185728
    Purpose
    Bacterial PAM profiling of adenine base editor variants
    Depositor
    David Liu
    Publication
    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038
    Inserts
    ecTadA(8e)-neNme2-C
    Nme2Cas9 sgRNA
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…
    Available since
    Sept. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    185728-plasmid-map-sequence-id-364088

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  8. pDAS12342_PEAR-GFP_2in1

    Plasmid
    #177184
    Purpose
    PEAR-GFP plasmid with a pegRNA that targets its own plasmid
    Depositor
    Ervin Welker
    Publication
    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    EGFP split with an intron between amino acids 95-96
    pegRNA targeting its own plasmid
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    disrupted 5' splice site
    Promoter
    CMV and U6
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    177184-plasmid-map-sequence-id-348471

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  13. pCF806-shABC2

    Plasmid
    #186714
    Purpose
    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC2 = shARAF.169-shBRAF.1296-shCRAF.387). The shABC2 set is better than shABC1.
    Depositor
    Christof Fellmann
    Publication
    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023
    Insert
    ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Available since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    186714-plasmid-map-sequence-id-382420

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  16. PX458-3xHA-SpCas9-HF1

    Plasmid
    #201952
    Purpose
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Publication
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201952-plasmid-map-sequence-id-405306

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  20. pB–DualPE

    Plasmid
    #235161
    Purpose
    For plant prime editing including large DNA fragment editing in wheat plants or other monocotyledons
    Depositor
    Zong Yuan
    Publication
    Zhao et al Nat Plants. 2025 Feb;11(2):191-205. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Csy4-P2A-Nls-nCas9-NC-nls-MLV-Nls
    CmYLCV-epegRNA-CaMV poly(A) signal
    Use
    CRISPR
    Mutation
    Detailed in manuscript
    Available since
    Oct. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    235161-plasmid-map-sequence-id-488642

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Showing: 9021 - 9040 of 15648 results