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pMPRMA45

Plasmid

#172934

Purpose

pQE30 encoding Wzz mutant i32

Depositor

Renato Morona

Publication

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i32 mutant gene

Tags

6xHis

Expression

Bacterial

Available since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMPRMA48

Plasmid

#172941

Purpose

pQE30 encoding Wzz mutant i131

Depositor

Renato Morona

Publication

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i131 mutant gene

Tags

6xHis

Expression

Bacterial

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMPRMA50

Plasmid

#172936

Purpose

pQE30 encoding Wzz mutant i219

Depositor

Renato Morona

Publication

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i219 mutant gene

Tags

6xHis

Expression

Bacterial

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b-sad1-C63A

Plasmid

#90302

Purpose

protein expression

Depositor

Christine Guthrie

Publication

Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

Insert

SAD1

Tags

His tag

Expression

Bacterial

Mutation

C63A

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_K227/230R_Keima

Plasmid

#127146

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3_Keima (RPS3 Human)

Use

Lentiviral

Mutation

K227R/K230R

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_K227/230R

Plasmid

#127135

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3 (RPS3 Human)

Use

Lentiviral

Mutation

K227R/K230R

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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TDK promoter dCas9

Plasmid

#113657

Purpose

dCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.

Depositor

Jeffrey Barrick

Publication

Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

Insert

TDK gene promoter and dCas9

Use

CRISPR

Expression

Bacterial

Promoter

TDK gene promoter

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAB085d7

Plasmid

#79211

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Publication

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) luxAB; Ppro1 434cI-TnCAD-F3

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAB085d6

Plasmid

#79210

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Publication

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) luxAB; Ppro1 434cI-TnTBR3-F3

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pM1GG

Plasmid

#108200

Purpose

M. sedula PCNA1

Depositor

Teruyuki Nagamune

Publication

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

M. sedula PCNA1

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFF19H

Plasmid

#49783

Purpose

expresses hygromycin resistance gene in plant cells

Depositor

Joachim Messing

Publication

Timmermans et al J Biotechnol. 1990 Jun;14(3-4):333-44.

Insert

HPH

Use

Plant expression

Promoter

CaMV 35S

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only
LI B-C N-NES KOZ b c7

Plasmid

#54445

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

Nuclear Export Signal (NES) of heat stable inhibitor (PKI)

Use

Cloning vector

Available since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA/Bmal W427A

Plasmid

#47349

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Publication

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

W427A

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA3-HA/Bmal V435R

Plasmid

#47350

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Publication

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

V435R

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pVRa37_3390

Plasmid

#49697

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF37_3390

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pVRa27_4265

Plasmid

#49682

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF27_4265

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pVRa20_2913

Plasmid

#49674

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF20_2913

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA/Bmal L150E

Plasmid

#47346

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Publication

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

L150E

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
K-p31

Plasmid

#38790

Depositor

David Wang

Publication

Siebrasse et al J Virol. 2012 Jun 27.

Insert

3742-4573 of MWPyV

Expression

Bacterial

Mutation

One SNP in comparison to MA095

Promoter

lac

Available since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only
pDMG8a

Plasmid

#33179

Depositor

David Bartel

Publication

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

C27H6.9 3'UTR (C27H6.9 Nematode)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only